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Table 1 Primers used in this study

From: Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes

Primer name Sequence
For defined library
   Up-BIO 5'-biotin-GAACTTCGAACTGCAGGTCGAC-3'
   Dn-BIO 5'-biotin-GTATAGGAACTTCGAAGCAGCTC-3'
   Up-Cy3 5'-GGTCGACGGACCCCCG-3'
   Up-Cy5 5'-GGTCAACGGATCCCCG-3'
   Dn-Cy3 5'-AAGCAGTTCCAGCCTACA-3'
   Dn-Cy5 5'-AAGCAGCTCCAGTCTACA-3'
For random library
   Tn10OE-BIO 5'-biotin-CAAGATGTGTATCCACCTTAACTTAA-3'
   Tn10OE OUT-Cy3 5'-ACCAATATCATTAGGGGAT-3'
   Tn10OE OUT-Cy5 5'-ACCAAAATCATAAGGGGAT-3'
Common for both libraries
   TATV-3 or Oligo(dT9 AT15V) 5'-T9 AT15V-3'
   TATV-5 or Oligo(dT15 AT9V) 5'-T15 AT9V-3'
  1. Mismatched nucleotides are in bold. 'V' represents A, C, or G.