Actual versus expected P values under uniform distribution for null genes of Latin Square 2× comparisons. (a) Actual versus expected P values for the comparison of experiment 10 versus 11. Black dashes indicate the y = x diagonal. (b) Box plots showing the results of the Kolmogorov-Smirnov test for each of the 14 Latin Square 2× comparisons under the null hypothesis that the observed distribution of P values was the same as a uniform distribution of P values. The red line is the P = 0.05 level. (c) Same data as in panel b but with a magnified y-axis.