Comparison of predictions from three methods on the ngLOC dataset. Three methods, PSORT, pTARGET, and ngLOC, were evaluated by comparing the Matthews Correlation Coefficient (MCC) for each localization. The MCC was chosen because it provides a balanced measure between sensitivity and specificity for each class . *The LYS location was omitted from PSORT predictions because PSORT predicts this class as part of the vesicular secretory pathway. CSK, cytoskeleton; CYT, cytoplasm; END, endoplasmic reticulum; EXC, extracellular; GOL, golgi; LYS, lysosome; MIT, mitochondria; NUC, nucleus; PLA, plasma membrane; POX, perixosome.