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Figure 6 | Genome Biology

Figure 6

From: On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation

Figure 6

Characterization of Arabidopsis 5' leader sequences. The analysis is based on a set of sequences obtained from cap-purified mRNAs (see Materials and methods). (a) Length distribution. (b) Number of uAUGs. (c) Correlation between length of the leader and number of uAUGs. (d) Distribution of uORF lengths among the 12,129 bona fide full-length leader sequences. uORFs that overlap the main ORF were not included in this analysis. (e) The frequency of each dinucleotide (AA, AC, and so on) was determined empiricially across all 5' leaders (not shown). Then, the theoretical frequency of each triplet was predicted based on the dinucleotide data (see Materials and methods for details) and set to 100%. The empirical frequency of each triplet across all 5' leaders was then expressed in relation to the predicted frequency.

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