Figure 1

Kinetics of the Ad5 infectious cycle in quiescent HFFs. Quiescent human foreskin fibroblasts (HFFs) were infected with 30 plaque forming units/cell adenovirus type 5 (Ad5) and total DNA or protein extracts prepared after various periods of infection, as described in Materials and methods. (a) Concentrations of viral DNA were determined by limiting polymerase chain reaction amplification of a sequence with the E1A gene. Purified Ad5 (Ad5) and human (Hu) DNAs were amplified as positive and negative controls, respectively. (b) The viral early E2 and late V proteins, and the cellular β-actin protein were examined by immunoblotting.