Year | Approach | Organelle | Number of proteins | Comment | Reference |
---|---|---|---|---|---|
1980 | Mutagenesis | Cell-wide | 23 | First attempt to systematically identify secretory machinery (S. cerevisiae) | [27] |
2000 | Proteomics | Golgi | 93 (588)* | First concerted effort to analyze the Golgi proteome. Cycloheximide treatment used to enrich for Golgi residents | [7] |
2003 | GFP-tagging | ER | 296 | Subcellular localization of more than 75% of the yeast proteome was determined using GFP fusions (S. cerevisiae) | [3] |
 |  | Golgi/TGN | 118 |  |  |
2004 | Proteomics | Golgi | 151 (421)†| Improved proteomics technologies allowed more comprehensive identification of proteins | [9] |
2006 | Functional assay | Cell-wide | 130 | First genome-wide RNAi screen to search for proteins with a functional involvement in secretion (Drosophila S2 cells) | [26] |
2006 | Proteomics | ER | 229 | Proteomics and protein correlation profiling of multiple organelles separated by gradient centrifugation | [14] |
 |  | ER-Golgi vesicles | 220 |  |  |
 |  | Golgi | 67 |  |  |
2006 | GFP-tagging | ER | 382 (68)‡ | Subcellular localization of 90% of the yeast proteome was determined using GFP fusions (S. pombe) | [4] |
 |  | Golgi | 156 (68)‡ |  |  |
2006 | Proteomics | ER | 832 (405)§ | Most quantitative proteomic characterization of the ER, Golgi and COPI vesicles reported to date | [13] |
 |  | Golgi | 193 (405)§ |  |  |