A spatially inconsistent set of alignments ('spider'). Black lines represent aligned supercontigs. Shaded regions between supercontigs correspond to alignments between supercontigs. This alignment conflict is indicative of a major misassembly (Figure 3a) in either supercontig 33,489 or 33,085. Genetic mapping revealed supercontig 33,489 to contain the misassembly, which was corrected by manually breaking it, retaining supercontigs 33,085 and 32,782, and the portion of supercontig 33,489 aligned to 33,085 (shaded gray) together, and placing supercontig 32,762 and the region of 33,489 aligned to 32,762 (shaded green) into a separate bin.