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Table 1 ICDSs shown by resequencing to correspond to sequencing errors in M. smegmatis mc2155

From: Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?

ICDS number 5' position ORF number Putative function Functional classification Accession number Type of event
0012 1639371 1547 Hypothetical Unknown DQ866846 U
0019 1918521 1842-1843 Adenosylhomocysteinase Intermediary metabolism DQ866847 U
0022 1930746 1854-1855 Sodium/proton antiporter Cell wall, process DQ866848 U
0024 2055797 1975-1976 Methane/phenol/toluene hydroxylase Intermediary metabolism DQ866849 O
0026 2119141 2042 Conserved hypothetical Unknown DQ866850 O
0027 2162020 2086-2087 Ferredoxin-NADP reductase Intermediary metabolism DQ866851 O
0028 2221312 2149-2150 Hypothetical Unknown DQ866852 U
0030 2290855 2215-2216 CoA-transferase Intermediary metabolism DQ866853 O
0035 2799279 2732-2733 Conserved hypothetical Unknown DQ866854 U
0039 3216877 3151 Aconitate hydratase Intermediary metabolism DQ866855 O (× 2)
0040 3262835 3192-3193 Maltooligosyltrehalose synthase Intermediary metabolism DQ866856 U
0041 3313327 3240 ABC transporter (CydC) Intermediary metabolism DQ866857 O
0051 3902349 3837 Dephospho-CoA kinase Intermediary metabolism DQ866858 O (× 2)
0053 3961899 3892-3893 Transcriptional regulator Regulation DQ866859 O
0054 4017126 3952-3953 Hypothetical Unknown DQ866860 O
0057 4255762 4183 Pyruvate dehydrogenase Intermediary metabolism DQ866861 U
0058 4288648 4211-4212 Nitrate reductase Intermediary metabolism DQ866862 U
0061 4637174 4539-4540 Oxidoreductase Intermediary metabolism DQ866863 O
0072 5644787 5533-5534 Hypothetical Unknown DQ866864 U
0073 5855980 5754 Acetyltransferase Intermediary metabolism DQ866865 O
0076 6078397 5970-5971 Fatty-acid CoA synthetase Lipid metabolism DQ866866 U
0080 6600510 6504-6505 Conserved hypothetical Unknown DQ866867 U
0082 6670969 6579 Helicase DNA metabolism DQ866868 O
0083 6673489 6581 Hypothetical Unknown DQ866869 U
0089 342400 * Methyltransferase Intermediary metabolism DQ866870 U
0091 601272 0511-0512 Hypothetical Unknown DQ866871 U
0092 809979 0716-0717 Transcriptional regulator Regulation DQ866872 U
0093 428949 1395-1396 Elongation factor G Translation DQ866873 O
  1. The nucleotide position, the affected ORF (according to the TIGR website), its putative function computed after the correction of the sequencing errors, its functional classification and its accession number are indicated for each ICDS. The asterisk indicates an ORF not predicted by TIGR. Two types of error were observed: overcall (O), an extra nucleotide not present in the target sequence was initially predicted at a given position; and undercall (U), a nucleotide corresponding to a true target sequence was not predicted at a given position.