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Table 2 Effects of the number and selection of IRCs on the increase in error and the fold difference between calibrated NRQs

From: qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

  Increase in error   Fold difference between calibrated normalized quantities  
  Mean [95% CI] Max Mean [95% CI] Max
1 IRC     
   run1-run2 1.684 [1.579,1.797] 10.98 1.048 [1.034,1.061] 1.143
   run1-run3 1.68 [1.576,1.79] 10.98 1.053 [1.038,1.067] 1.135
2 IRCs     
   run1-run2 1.374 [1.289,1.466] 7.73 1.024 [1.017,1.03] 1.069
   run1-run3 1.489 [1.415,1.567] 7.73 1.026 [1.019,1.033] 1.065
3 IRCs     
   run1-run2 1.399 [1.292,1.513] 5.28   
   run1-run3 1.394 [1.288,1.508] 5.28