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Table 2 Effects of the number and selection of IRCs on the increase in error and the fold difference between calibrated NRQs

From: qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

 

Increase in error

 

Fold difference between calibrated normalized quantities

 
 

Mean [95% CI]

Max

Mean [95% CI]

Max

1 IRC

    

   run1-run2

1.684 [1.579,1.797]

10.98

1.048 [1.034,1.061]

1.143

   run1-run3

1.68 [1.576,1.79]

10.98

1.053 [1.038,1.067]

1.135

2 IRCs

    

   run1-run2

1.374 [1.289,1.466]

7.73

1.024 [1.017,1.03]

1.069

   run1-run3

1.489 [1.415,1.567]

7.73

1.026 [1.019,1.033]

1.065

3 IRCs

    

   run1-run2

1.399 [1.292,1.513]

5.28

  

   run1-run3

1.394 [1.288,1.508]

5.28