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Table 2 Effect of filter threshold setting and LPI score ranking on eukaryotic BLAST matches to E. coli

From: DarkHorse: a method for genome-wide prediction of horizontal gene transfer

Filter threshold

Query id

Match id

LPI

Percent identity

Query length

Align length

e-value

Bit score

Match species

Query annotation

Match annotation

0.0

AAC74689

CAC43289

0.009

99

603

603

0

1261

Arabidopsis thaliana

Beta-glucuronidase

Beta-glucuronidase

0.02

AAC74689

ZP_00698534

0.981

99

603

603

0

1255

Shigella boydii

 

Beta-galactosidase/beta-glucuronidase

0.0

AAC76624

AAM52982

0.009

99

382

382

0

741

Dunaliella bardawil

Mannitol-1-phosphate dehydrogenase

Mannitol-1-phosphate dehydrogenase

0.02

AAC76624

AAN45081.2

0.981

98

382

382

0

738

Shigella flexneri

 

Mannitol-1-phosphate dehydrogenase

0.0

AAC73440

AAU04862

0.001

96

427

425

0

830

Tamarix chinensis

Cytosine deaminase

Cytosine deaminase

0.2

AAC73440

AAV79026

0.925

81

427

420

0

706

Salmonella enterica

 

Cytosine deaminase

0.0

AAC73353

AAA35359

0.088

78

155

99

7.0E-42

171

Cercopithecus aethiops

CP4-6 prophage

None

0.2

AAC73353

ZP_00825492

0.924

48

155

145

1.0E-36

153

Yersinia mollaretii

 

Hypothetical protein

0.0

AAC75891

gi|2143952

0.108

85

458

441

0

719

Rattus norvegicus

Predicted transcriptional regulator

Hepatic glutathione transporter

0.8

AAC75891

AAD12579

0.927

28

458

403

1.0E-38

164

Salmonella typhimurium

 

HilA

0.0

AAC73796

BAB33410

0.029

100

108

108

1.0E-54

213

Pisum sativum

Predicted inner membrane protein

Putative senescence-associated protein

0.0

AAC74583

BAE25662

0.104

92

1325

895

0

1614

Musmusculus

Predicted lipoprotein

none

0.0

ABD18679

gi|1095170

0.108

93

234

179

3.0E-86

320

Rattus norvegicus

Predicted protein, amino terminal fragment (pseudogene)

Glutathione transporter

  1. Rows in bold type contain the top ranked match using a zero threshold setting. Rows in italic type show cases where using a higher filter setting revealed an alternative match, with a higher LPI score, to the same genomic query.