Transcriptional profiling reveals barcode-like toxicogenomic responses in the zebrafish embryo

Table 1 Summary of microarray experiments

Toxicants	Stage	Concentration	Arrays
4CA	24 hpf	15 ppm 15 mg/l 118 μM	8 (3)
	48 hpf	50 ppm 50 mg/l 390 μM	6 (3)
	120 hpf	50 ppm 50 mg/l 390 μM	8 (3)
	120 hpf	25 ppm 25 mg/l 195 μM	4 (1)
	120 hpf	5 ppm 5 mg/l 39 μM	4 (1)
	120 hpf	0.5 ppm 0.5 mg/l 3.9 μM	4 (1)
DDT	24 hpf	5 ppm 5 mg/l 14 μM	6 (3)
	48 hpf	15 ppm 15 mg/l 42 μM	6 (2)
	120 hpf	15 ppm 15 mg/l 42 μM	8 (3)
	120 hpf	1.5 ppm 1.5 mg/l 4.2 μM	4 (1)
	120 hpf	0.15 ppm 0.15 mg/l 0.42 μM	4 (1)
Cd	24 hpf	0.5 ppm 0.5 mg/l 2.7 μM	8 (4)
	48 hpf	5 ppm 5 mg/l 27 μM	8 (3)
	120 hpf	5 ppm 5 mg/l 27 μM	8 (3)
	120 hpf	2.5 ppm 2.5 mg/l 13.5 μM	4 (2)
	120 hpf	0.5 ppm 0.5 mg/l 2.7 μM	4 (2)
	120 hpf	50 ppb 50 μg/l 0.27 μM	4 (2)
TCDD	24 hpf	150 ppt 150 ng/l 0.47 nM	8 (3)
	48 hpf	500 ppt 500 ng/l 1.6 nM	4 (2)
	120 hpf	500 ppt 500 ng/l 1.6 nM	8 (3)
	120 hpf	250 ppt 250 ng/l 0.8 nM	4 (1)
	120 hpf	50 ppt 50 ng/l 0.16 nM	4 (2)
VA	24 hpf	15 ppm 15 mg/l 12.9 μM	8 (3)
	48 hpf	50 ppm 50 mg/l 43 μM	8 (3)
	120 hpf	50 ppm 50 mg/l 43 μM	8 (3)
	120 hpf	25 ppm 25 mg/l 21.5 μM	4 (1)
	120 hpf	5 ppm 5 mg/l 4.3 μM	4 (1)
	120 hpf	0.5 ppm 0.5 mg/l 0.43 μM	4 (1)
MeHg	24 hpf	50 ppb 50 μg/l 0.20 μM	8 (3)
	48 hpf	60 ppb 60 μg/l 0.24 μM	6 (2)
	120 hpf	60 ppb 60 μg/l 0.24 μM	10 (3)
	120 hpf	30 ppb 30 μg/l 0.12 μM	4 (2)
	120 hpf	6 ppb 6 μg/l 0.024 μM	4 (2)
As	120 hpf	79 ppm 79 mg/l 400 μM	8 (3)
	120 hpf	7.9 ppm 7.9 mg/l 40 μM	4 (1)
Pb	120 hpf	2.8 ppm 2.8 mg/l 10 μM	8 (3)
	120 hpf	0.28 ppm 0.28 mg/l 1 μM	4 (1)
PCB	120 hpf	33 ppm 33 mg/l 100 μM	8 (3)
AA	120 hpf	71 ppm 71 mg/l 1 mM	8 (3)
tBHQ	120 hpf	1.7 ppm 1.7 mg/l 10 μM	8 (3)
Mixture	120 hpf	Pb 1 μM, Cd 0.27 μM, As 40 μM, Hg 0.024 μM	6 (3)

Embryos were either treated from 4 to 24 (24 hpf) or from 24 to 48 hpf (48 hpf) or from 96 to 120 hpf (5 days). Arrays, total number of microarray hybridizations. Numbers in brackets indicate the number of independent biological repeats. 4CA, 4-chloroaniline; DDT, 1,1-bis-(4-chlorphenyl)-2,2,2-trichlorethane; Cd, cadmium chloride; TCDD, 2,3,7,8-tetrachlorodibenzo-p-dioxin; VA, valproic acid; MeHg, methylmercury chloride; As, arsenic (III) oxide; Pb, lead (II) chloride; AA, acrylamide, PCB, Aroclor 1254; tBHQ, tert-butylhydroquinone.

ISSN: 1474-760X