Figure 6

The repair proteins RadA and SSB are not strongly induced by UV irradiation. Western blot using antibodies against S. solfataricus RadA and SSB. Cell-free extracts were prepared from samples taken from UV-treated and control cultures at the indicated time points. In agreement with the microarray data, RadA levels do not increase significantly following UV irradiation. The levels of SSB protein are also largely unchanged, in agreement with quantitative RT-PCR (Table 2), though the microarray data showed a modest rise in SSB transcription at early time points after UV damage.