Figure 1

Meiotic gene expression program in rep1Δ and mei4Δ mutants. (a) Left: hierarchical cluster analysis with columns representing experimental time points and rows representing early genes. Vegetatively growing cells (V) are synchronized in G1 by nitrogen removal and enter meiosis by temperature shift at time 0. The mRNA levels at each time point of pat1(from [4]) and rep1Δ pat1 time courses relative to the levels in vegetative cells are color-coded as indicated at the bottom with missing data in gray. Rep1p-independent and -dependent genes are indicated at right as drawn-out or dashed blue lines, respectively. Right: average expression profiles of Rep1p-dependent and Rep1p-independent genes in wild-type (pat1-synchronized cells from [4]) and rep1Δ cells. (b) Average expression profiles of the four main gene clusters upregulated during pat1-induced meiosis: nitrogen-starvation response (black), early (blue), middle (red) and late (green), as defined in [4]. Experimental details are as in (a). The y-axis shows the expression level at the corresponding time point relative to expression in vegetative cells. Graphs from left to right: pat1 cells (from [4]) and mei4Δ pat1 cells. (c) Hierarchical cluster analysis with columns representing experimental time points and rows representing genes. The mRNA levels at each time point of pat1 time courses relative to the levels in vegetative cells are color-coded as indicated in (a). The last column (OE) shows the expression level in cells overproducing Mei4p relative to an empty vector control. The four gene clusters are indicated on the left.