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Table 2 Combinatorial RNAi can identify known synthetic lethal interactions

From: Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution

Strain Interaction Gene1 + Gene 2 Gene1 Gene2 Gene1 + 2 Syn p value
   BS ES BS ES BS ES   BS ES
Wild-type mec-8 + sym-1 88 99 82 98 78 92 Yes 5.5 × 10-01 1.3 × 10-02
  sop-3 + sop-1 91 100 94 99 79 90 Yes 2.8 × 10-01 8.5 × 10-04
  tbx-8 + tbx-9 83 99 78 97 52 11 Yes 7.3 × 10-02 1.4 × 10-24
  hlh-1 + unc-120 91 99 76 99 28 91 Yes 5.2 × 10-05 1.2 × 10-02
  hlh-1 + hnd-1 88 97 75 98 62 81 Yes 6.6 × 10-01 5.7 × 10-03
  unc-120 + hnd-1 54 100 74 98 36 100 No 6.4 × 10-01 1.9 × 10-01
  egl-27 + egr-1 93 99 79 90 90 89 No 6.0 × 10-02 7.4 × 10-01
rrf-3 mec-8 + sym-1 67 73 61 73 59 16 Yes 3.3 × 10-01 3.0 × 10-06
  sop-3 + sop-1 82 100 85 96 41 75 Yes 3.1 × 10-04 5.7 × 10-06
  tbx-8 + tbx-9 96 99 86 92 59 2 Yes 8.6 × 10-03 6.3 × 10-27
  hlh-1 + unc-120 90 90 31 99 1 64 Yes 8.1 × 10-06 2.9 × 10-03
  hlh-1 + hnd-1 86 87 82 94 42 24 Yes 1.6 × 10-03 8.2 × 10-14
  unc-120 + hnd-1 33 100 87 94 7 98 Yes 5.7 × 10-04 4.8 × 10-02
  egl-27 + egr-1 97 99 83 93 73 62 Yes 2.9 × 10-01 5.7 × 10-08
  1. Quantitative analysis of known synthetic lethal interactions (Interaction Gene1 + Gene2; see below for references) after combinatorial RNA interference (RNAi) in wild-type or RNAi-hypersensitive rrf-3 worms [19]. Percentages of average wild-type brood size (BS) and embryonic survival (ES) rates resulting from RNAi targeting each gene individually (Gene1 or Gene2) as well as targeting both genes simultaneously (Gene1 + 2) are shown. A synthetic interaction (Syn) was scored positive for p < 5.0 × 10-02 (by Student's t-test). References for genes tested: mec-8 + sym-1 [21]; sop-3 + sop-1 [22]; tbx-8 + tbx-9 [23]; hlh-1 + unc-120, hlh-1 + hnd-1, unc-120 + hnd-1 [24]; and egl-27 + egr-1 [25].