Figure 1

An interaction network of proteins involved in spinocerebellar ataxias. The yeast two-hybrid interaction data of Lim et al. [1] reveal one large interconnected network consisting of 752 protein-protein interactions between 36 ataxia-associated proteins and 541 prey proteins. Circles (nodes) represent proteins, and any two proteins connected by a line have been shown to interact in the yeast two-hybrid (Y2H) screen. Blue circles depict protein baits corresponding to the proteins known to be mutated in ataxias; red circles depict protein baits that are paralogs of ataxia-causing proteins or known interactors with them. The yellow circles depict prey proteins tested in the yeast two-hybrid screen and come from two sources. Those connected by a purple line to a node come from the human open reading frame library (the hORFeome), while those connected by a green line come from a human brain cDNA library. All lines represent either first- or second-order interactions to ataxia-causing proteins. First-order interactions are direct interactions, while second-order interactions occur via an intermediary protein. Reproduced with permission from Elsevier [1].