Figure 2

Novel gene annotation. This yrGATE implementation at ZmGDB presents the region 158659-162032 of Zea mays BAC gi 51315585. (a) The main Annotation Tool window contains a completed gene structure annotation. The provided transcript evidence consists of two groups of ESTs (9, circled) separated by a region with no spanning evidence, 160260-160664 (8). User defined exons have been designated in this region. The User Defined Exons Table (2) lists each exon by coordinates and source. (b) Exon 5, 160575..160721, was defined using portals to (b) GENSCAN and GeneSeqer@PlantGDB (not shown). Yellow buttons in the GENSCAN portal (b) add exons to the gene structure in the Annotation Tool (6 in panel a), which are presented pictorially (10 in panel a) for comparison with the Evidence Plot. A protein-coding region was evaluated using the portal to the (c) ORF Finder and imported into the Annotation Tool (4 in panel a) using the yellow button.