Table 3 Primer sequences used in real-time RT-PCR
From: Signatures of human regulatory T cells: an encounter with old friends and new players
Gene | Primers |
|---|---|
FOXP3 | 5'-GAA CGC CAT CCG CCA CAA CCT GA-3' |
| Â | 5'-CCC TGC CCC CAC CAC CTC TGC-3' |
CTLA4 | 5'-TGC AGC AGT TAG TTC GGG GTT GTT-3' |
| Â | 5'-CTG GCT CTG TTG GGG GCA TTT TC-3' |
CCR7 | 5'-TGG CCT GCA GGA AAC ACC-3' |
| Â | 5'-GGG AGA CTT CTT GGC TTG GTG AG-3' |
RPS9 | 5'-CGC AGG CGC AGA CGG TGG AAG C-3' |
| Â | 5'-CGA AGG GTC TCC GCG GGG TCA CAT-3' |
TNFRSF1B | 5'-GTA GCC TTG CCC GGA TTC TGG-3' |
| Â | 5'-ACC CTG CCC CTG CTC TGC TA-3' |
TRAF1 | 5'-GGG GCA TAA ACT TTC CTC TTC C-3' |
| Â | 5'-TTT GGG GTT ATA CAT TGC TCA GTG-3' |
LGALS3 | 5'-CCT TTG CCT GGG GGA GTG GTG-3' |
| Â | 5'-TGA AGC GTG GGT TAA AGT GGA AGG-3' |