Figure 6
From: Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system
Parameters affecting the degree of proteome coverage. The dark blue terms pertain to the characteristics of the mass spectrometer and associated on-line chromatography. In red are the corresponding characteristics of the proteome. The blue arrows indicate that the three parameters are interdependent. For example, limited dynamic range and sequencing speed act together to reduce the effective sensitivity in complex mixtures to below that of single proteins.