| | Preys |
|---|
Baits | Aco1 | Aha1 | Aro1 | Cct8 | Gfa1 | Pfk1 | Pfk2 | Yhb1 | Additional |
|---|
Aco1 | - | - | - | - | - | - | - | - | - |
Aha1 | - | x | x | - | x | x | - | - | +26 |
Aro1 | - | - | x | x | x | x | - | x | +24 |
Cct8 | - | - | - | - | - | - | - | - | - |
Gfa1 | - | - | - | - | - | - | - | - | - |
Pfk1 | - | - | - | - | - | - | - | - | - |
Pfk2 | - | - | - | - | - | x | x | - | +3 |
Yhb1 | x | - | - | - | x | - | - | x | +18 |
Prey count | 10 | 7 | 17 | 10 | 106 | 30 | 21 | 13 | |
- Purifications that lead to the definition of complex 314 as described by Gavin et al. [1] and in Figure 1e. Each line represents a single purification with the bait indicated. For each of the bait proteins, the columns indicate the prey proteins (x) associated with the bait or not found with the bait (-). Complex 314 consists of the core proteins Aro1 and Cct8, which were found in two purifications of which only one (bait: Aro1) is shown here. Aco1 was found in 10 purifications, that is, in 9 purifications in addition to the one using Yhb1 as bait. Only baits that were included in the 'final' complex by the SAI algorithm are shown in this table. In addition, the modules 103 (Pfk1-Pfk2, found in five purifications) and 114 (Aco1-Yhb1, found in three purifications) associated with the core, as indicated by the co-purification of Pfk2 with Pfk1 and of Aco1 with Yhb1. Finally, Aha1 and Gfa1 were classified as attachments because they were not found consistently associated with any of the other components and thus could not be classified as core or module. Note that Aha1, Aro1 and Yhb1 had many more proteins co-purified when they were used as baits than when they were prey. For example, when Aha1 was used as a bait, the four proteins Aha1, Aro1, Gfa1 and Pfk1 were identified as binding to it, plus another 26 proteins not shown here; these are indicated in the Additional column. Purifications with Aco1, Cct8, Gfa1 and Pfk1 as baits appear to have been unsuccessful. Note that the information in this table is not sufficient to derive the complex shown in Figure 1e but also requires information from additional purifications only indicated in the row 'Prey count' and in the column 'Additional'.
