Figure 2

Protein complexes, purification data and two-hybrid interactions. Protein complex 285 from Krogan et al. [2] (composed of Spc72, Spc97 and Tub4 - the proteins in the area shaded yellow) was compared with complex 219 from Gavin et al. [1] (composed of all the proteins in the yellow and pink areas). Note that the two 'complexes' have been derived from many purifications by computational means and do not necessarily represent physical entities. The purifications from [1] using Spc97 and Tub4 as baits did not produce completely overlapping prey sets (there was no purification reported with Spc72 as bait); for example, only Tub4 but not Spc97 co-purified with Tef1 and Pfk1, whereas Spc97 but not Tub4 pulled down Spc72. Independently, two-hybrid screens have found a number of interactions between the members of these complexes and with other proteins, as indicated by arrows (pointing from the bait to the prey). The two-hybrid data are from [11,18-20].