Example of intron loss. Multiple alignment of the two duplicated rice genes (top; LOC__Os03g18690.1, LOC_Os07g49150.1) and their putative orthologous Arabidopsis gene (bottom; At4g29040.1) suggests that the third intron of LOC_ Os07g49150.1 was lost. Yellow inserts indicate conserved introns across the three genes while red indicates lost intron. The phase of the intron is inserted into the alignment. All conserved introns are phase 0 whereas the lost intron is phase 2. The two rice genes and putative Arabidopsis ortholog encode a 26S proteasome regulatory subunit 4.