Figure 2From: RNA interference is not involved in natural antisense mediated regulation of gene expression in mammalsSingle cell RNA expression of TS transcripts. (a) Antisense probe; (b) sense probe; (c) both sense and antisense probes bound to the fixed and reverse transcribed TS RNA in HeLa cells. Probes were designed to cover exon boundaries and a part of the overlap region in a strand specific manner. (d) Signals from the actin probe show that the method was working optimally. All the probes were intron spanning to avoid background signal from contaminating DNA.Back to article page