Figure 2
![Figure 2](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fgb-2006-7-5-r38/MediaObjects/13059_2005_Article_1306_Fig2_HTML.jpg)
Single cell RNA expression of TS transcripts. (a) Antisense probe; (b) sense probe; (c) both sense and antisense probes bound to the fixed and reverse transcribed TS RNA in HeLa cells. Probes were designed to cover exon boundaries and a part of the overlap region in a strand specific manner. (d) Signals from the actin probe show that the method was working optimally. All the probes were intron spanning to avoid background signal from contaminating DNA.