Figure 1
From: Ranked prediction of p53 targets using hidden variable dynamic modeling
Model based estimation of activity profile of p53. (a) Markov Chain Monte Carlo output for potential transcription factor activity profile values for first time series replicate at 4 hours (x axis) and 6 hours (y axis). (b) Concentration of p21WAF1 transcript determined by real-time polymerase chain reaction after addition of actinomycin D (10 μg/ml) to irradiated (5 Gy, 4 hours) MOLT4 cells cultured in RPMI. Expressed as percentage of initial concentration. (c) Using the degradation rate of p21WAF1 dramatically restricted the range of solutions to the Markov Chain Monte Carlo.