Anopheles gambiae genome reannotation through synthesis of ab initioand comparative gene prediction algorithms

Table 1 Comparison of ReAnoCDS05 and Ensembl

	ReAnoCDS05	Ensembl
Total CDSs	31,254	16,148
Total exons	155,680	58,579
Average exons per CDS	4.98	3.62
CDS completion rate*	96%	37%
CDSs overlapped by cDNA pair contigs^†	1,885	2,257
CDSs perfectly matched to cDNA pair contigs^†	752	672
cDNA pair contigs not overlapping any CDSs^†	1%	8%
Specificity (nucleotide)^‡	0.96	0.99
Sensitivity (overall)^§	99%	92%
Sensitivity (perfect)^¶	45%	30%
Protein hits yielded by MS peptides	4,737	1,413
MS peptide missing rate^¥	12%	62%

*Proportion of CDSs with start and stop codon. ^†Paired end sequences of full-length cDNAs from [7]. ^‡Calculation for ReAnoCDS05 described in Materials and methods, Ensembl value from [18]. ^§Proportion of cDNA pair contigs overlapping a CDS. ^¶Proportion of overlapped CDSs precisely matching cDNA pair contig boundaries. ^¥Proportion of mass spectrometry (MS) peptides failing to hit any protein in database.

ISSN: 1474-760X