Phylogenetic and structural analysis of centromeric DNA and kinetochore proteins

Table 1 Sequence similarities among selected fungal kinetochore proteins of point CEN

Location	Complex	Protein	Ubiquitous	Point CEN specific	Similarity*	Identity*
DNA-binding	Monomer	Mif2	+		65%	23%
	?	Sgt1	+		74%	28%
	CBF3	Cep3		+	65%	14%
		Ctf13		+	53%	9%
		Ndc10		+	48%	10%
Linker layer	COMA	Mcm21	+		45%	7%
		Ctf19	+		47%	7%
		Ame1		+	45%	9%
		Okp1		+	51%	7%
	MIND	Nnf1	+		67%	14%
		Nsl1	+		69%	15%
	NDC80	Ndc80	+		73%	20%
		Spc24	+		63%	6%
	SPC105	Spc105	+		48%	5%
		Ydr532C		+	52%	6%
	?	Chl4	+		52%	11%
	?	Ctf3	+		51%	6%
	?	Nkp1^†		+	55%	6%
	?	Nkp2^†		+	63%	6%
	?	Mcm16		+	52%	7%
	?	Mcm22		+	53%	4%
	?	Iml3^‡	+		24%	6%
	?	Cnn1		+	40%	4%
MT-binding	DASH	Ask1	+		43%	11%
		Dam1	+		54%	6%
Regulatory	?	Bub3	+		65%	18%
	?	Mad2	+		98%	54%

*As determined from the proteins in S. cerevisiae, C. glabrata, E. gossyppii and K. lactis. ^†Instead of E. gossypii the sequences were derived from the very closely related S. kluyveri. ^‡Similarity was determined from proteins of the point CEN containing S. cerevisiae, S. kudriavzevii, K. waltii and S. kluyveri.

ISSN: 1474-760X