Figure 2

Phylogenetic profiling of novel nucleolar/ribosome-associated proteins. Phylogenetic profiles of 62 previously unrecovered nucleolar/ribosome-associated proteins of yeast across 84 organisms. The profiles were generated using the best reciprocal hit method with yeast as a reference organism (see Materials and methods). Abbreviations given on the top of the plot represent organism names (first three letters for genus and first three letters of species names; see Materials and methods for a translation of abbreviations into organism names). Further taxonomic annotation is given on the bottom of the plot. Yeast open reading frame identifiers are given on the left side, and gene names and descriptions are given on the right side of the plot. The significance of sequence similarity is visualized by different shades of gray that reflect the logarithmic expectation (E) value from reciprocal BLAST searches (shown at the bottom of the figure). Here, the E values of BLAST searches using target proteome sequences as queries versus the yeast proteome reference database are shown. The genes are ordered according to hierarchical clustering (see Materials and methods).