Figure 1

Sensitivity and development of tolerance to alcohol in Drosophila melanogaster. (a) Inebriometer elution profiles of Canton S B and Canton S E flies after exposure to concentrated ethanol vapor. Note the shifts in peak elution time between the first (solid symbols) and second (open symbols) ethanol exposure, and the difference in this peak shift between Canton S B and Canton S E. (b) Schematic diagram of the experimental design used to assess whole genome transcriptional changes after ethanol exposure. The numbers indicate samples collected for microarray analysis, as follows: 1, control flies; 2, flies after a single exposure to ethanol; 3, flies that develop tolerance during a 2 h interval prior to a second exposure to ethanol.