Figure 2

The two approaches discussed in this article for identifying the biosynthetic pathway of patellamide and expressing it in an alternative host bacterium. (a) Schmidt and colleagues [5] used an approach of complete genome sequencing, followed by sequence analysis to identify the biosynthetic pathway, cloning of the pathway into a heterologous host, and isolating the small molecule. (b) Jaspars and colleagues [6] used shotgun cloning of genomic DNA followed by screening of the resulting clone library for patellamide production. These steps could, in principle, be followed by sequencing the pathway, a step not reported by Jaspars and colleagues [6].