MicroRNA profiling of the murine hematopoietic system

Table 2 Correlation between arrays and Northern blot data

		DNT	Pro-B	Spleen B	BMMC	Hippocampus
MiR 7	Arrays	+	++	++	+/-	++
	Northerns	+	+++	++	n.a.	n.a.
MiR 24	Arrays	+++	++++	+++	++	++
	Northerns	++	++++	+++	n.a.	n.a.
MiR 26a	Arrays	+	+	++	+++	+++
	Northerns	+	++	+++	n.a.	n.a.
MiR 29a	Arrays	+	+	++	++	+++
	Northerns	+	+	++	nd	+++
MiR 93	Arrays	++	++	+/-	++	+/-
	Northerns	+	++	+	n.a.	n.a.
MiR 99a	Arrays	n.s.s.	n.s.s.	n.s.s.	n.s.s.	n.s.s.
	Northerns	+/-	+/-	-	nd	nd
MiR 101	Arrays	++	+++	+	+++	++
	Northerns	+	+	+	nd	++
MiR-107	Arrays	+++	+++	++	++	++
	Northerns	+	++	+	n.a.	n.a.
MiR-127	Arrays	+/-	+	+	+/-	+
	Northerns	-	-	-	n.a.	n.a.
MiR-142-3p	Arrays	+++	+++	++++	+++	+
	Northerns	++	+++	++++	++	n.a.
MiR-142-5p	Arrays	++	+	+++	++	+
	Northerns	+	++	+++	+	n.a.
MiR-144	Arrays	n.s.s.	n.s.s.	n.s.s.	n.s.s.	n.s.s.
	Northerns	-	-	-	n.a.	n.a.
MiR-148	Arrays	+	+	+	+	+/-
	Northerns	+	+	+	n.a.	n.a.
MiR-150	Arrays	-	+	+++	+	+/-
	Northerns	-	-	+++	n.a.	-
MiR-181b	Arrays	+	+	+	+	++
	Northerns	+	+	-	n.a.	+
MiR-191	Arrays	+/-	+	+	+	+
	Northerns	+	++	++	n.a.	n.a.
MiR-199	Arrays	+	+	+	+	+/-
	Northerns	-	+	-	n.a.	n.a.
MiR-206	Arrays	++	+++	+	+/-	+/-
	Northerns	-	-	-	-	n.a.
MiR-213	Arrays	n.s.s.	n.s.s.	n.s.s.	n.s.s.	n.s.s.
	Northerns	-	+/-	-	n.a.	n.a.
MiR-223	Arrays	+	+++	+	+/-	+
	Northerns	+/-	-	+	+	n.a.
MiR-342	Arrays	+	+	+	+	+
	Northerns	+	+++	++	n.a.	n.a.

The table summarizes and compares the Northern blot data shown in Figure 4 and the array data shown in the heat map in Figure 5. Northern blot and array data were scored independently using an arbitrary scale from undetectable (-) to strongly detected (++++) to indicate relative signal intensity in each case. BMMC, bone marrow-derived mast cell; DN T, double-negative thymocyte; n.s.s., non statistically significant hybridization signal; n.a., not analyzed.

ISSN: 1474-760X