Figure 3

Microarrays and Northern blots correlate qualitatively and quantitatively. Northern blots for miRNA expression in mast cells (left panels), or T cells (right panels). BMMC were treated with cyclosporin A or PMA and ionomycin for the indicated amounts of time. Loading control is the same as Figure 2d. First row underneath the panels: ratio between the intensities of the Northern blot bands as assessed by phosphorimager and quantified by ImageQuant; all the samples are compared with Pu.1-/- cells except for miR-223, where each sample is compared with BMMC. Second row: these are also ratios between the intensities of the Northern blot bands, but the T cell samples are compared directly to each other. This allows a better direct comparison with the numbers on the third row, which are the ratios of BMMC versus Pu.1-/- cells (left panels) and D5 versus D10 versus naïve T cells as obtained from the arrays (right panels). BMMC, bone marrow-derived mast cells; CsA, cyclosporin A; n.d., not detectable.