Figure 3

PCR analysis of putative macaque-specific sequences. PCR primers were developed from high-quality macaque cDNA sequences - (a) CX078602, (b) CX078598, and (c) CB555845 - and used to test for the presence or absence of the resulting amplicons in genomic DNA from 12 primate genomes, including two separate humans. Amplification conditions were the same as in Materials and methods, except that annealing was performed at 55°C. Expected product sizes are as in Table 6. (d) Amplification primers from exon 4 of the human oligoadenylate synthetase 1 gene (OAS1 ) are included as a positive control, resulting in the expected 648-bp product from most primate species.