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Table 9 Quantitative real-time PCR results for selected chromosome 21 genes in Table 1

From: Primary and secondary transcriptional effects in the developing human Down syndrome brain and heart

Gene name Chromosome p value Cerebrum Cerebellum Astrocyte Heart
    Microarray qPCR Microarray qPCR Microarray qPCR Microarray qPCR
Pituitary tumor-transforming 1 interacting protein (PTTG1IP) 21 1.50E-07 1.52 3.85 ± 0.38 1.42 1.46 ± 0.62 1.65 2.42 ± 1.16 1.74 1.41 ± 0.16
ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (ATP5O) 21 5.11E-07 1.69 1.52 ± 0.20 1.75 0.91 ± 0.16 1.34 1.48 ± 0.72 1.69 3.52 ± 1.22
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 (ATP5J) 21 2.47E-06 1.84 1.25 ± 0.14 1.40 1.19 ± 0.12 1.51 1.73 ± 0.78 2.23 5.19 ± 1.60
Down syndrome critical region gene 3 (DSCR3) 21 1.44E-05 1.82 2.76 ± 0.44 1.86 0.79 ± 0.38 3.40 2.50 ± 0.62 0.98 2.06 ± 0.06
Chromosome 21 segment HS21C048, zinc finger protein 294 (ZNF294) 21 3.39E-05 1.71 2.58 ± 0.24 1.42 1.30 ± 0.42 1.63 1.60 ± 0.24 1.66 1.51 ± 0.14
Superoxide dismutase 1 (SOD1) 21 5.62E-05 2.12 1.72 ± 0.24 1.57 1.44 ± 0.12 1.55 2.75 ± 1.37 1.74 2.86 ± 1.52
Cystatin B (stefin B) (CSTB) 21 7.75E-05 1.69 1.21 ± 0.13 1.49 1.21 ± 0.14 1.18 1.92 ± 0.56 1.30 1.88 ± 0.07
Phosphofructokinase, liver (PFKL) 21 1.93E-04 1.16 2.17 ± 0.14 1.92 1.35 ± 0.08 1.61 2.07 ± 0.44 1.11 2.23 ± 0.55
Pyridoxal (pyridoxine, vitamin B6) kinase (PDXK) 21 2.82E-04 2.73 2.99 ± 0.40 2.13 1.79 ± 0.20 2.21 2.18 ± 1.01 1.44 1.75 ± 0.09
Collagen, type VI, alpha 1 (COL6A1) 21 5.04E-04 1.72 3.16 ± 0.24 1.39 0.75 ± 0.06 1.45 2.57 ± 1.28 1.39 1.97 ± 0.76
Ubiquitin specific protease 16 (USP16) 21 5.33E-04 1.80 2.75 ± 0.48 1.30 1.33 ± 0.36 1.67 1.70 ± 0.50 1.67 3.40 ± 1.54
SMT3 suppressor of mif two 3 homolog 1 (yeast) (SMT3H1) 21 6.27E-04 1.68 1.66 ± 0.42 1.50 0.93 ± 0.08 1.56 2.56 ± 1.26 1.40 4.07 ± 2.71
Mitochondrial ribosomal protein L39 (MRPL39) 21 7.48E-04 1.44 1.25 ± 0.08 1.04 1.40 ± 0.27 1.24 2.41 ± 1.14 1.41 1.91 ± 0.29
Interferon gamma receptor 2 (IFNGR2) 21 8.16E-04 1.36 2.21 ± 0.29 1.36 1.78 ± 0.12 1.48 1.82 ± 0.44 1.27 1.27 ± 0.41
Human homolog of ES1 (zebrafish) protein (C21orf33) 21 1.02E-03 1.48 5.44 ± 2.14 1.71 1.85 ± 0.40 1.06 1.21 ± 0.24 1.99 1.38 ± 0.16
Chaperonin containing TCP1, subunit 8 (theta) (CCT8) 21 1.45E-03 1.38 2.51 ± 0.44 1.29 3.64 ± 0.92 1.94 1.09 ± 0.07 1.77 0.75 ± 0.04
Tryptophan rich basic protein (WRB) 21 2.18E-03 1.89 5.21 ± 2.08 1.28 1.94 ± 0.39 1.83 2.87 ± 2.23 1.94 1.78 ± 0.44
HMT1 hnRNP methyl-transferase-like 1 (HRMT1L1) 21 3.62E-03 1.37 1.50 ± 0.54 1.44 1.93 ± 0.36 0.96 1.46 ± 0.50 1.45 1.65 ± 0.05
  1. Data were normalized to the HPRT housekeeping gene. P values were derived from ANOVA of microarray data. Values for microarray data are fold-regulation. For each quantitative PCR (qPCR) experiment, values were determined by measuring samples in duplicate at multiple concentrations (mean ± standard error). Each experiment was performed independently at least three times.