Mutations in brat lead to defects in genetic and biological responses to 20E. (a) Control w1118pharate adult. (b-d) Representative bratk06028mutant animals. (e) Northern blot analysis of w1118and bratk06028mutants staged in hours relative to pupariation. Blots were probed to detect brat mRNA and transcripts from seven different 20E-regulated genes. Hybridization to detect rp49 mRNA was included as a control for loading and transfer.