Microarray results for EcRi and 20E organ cultures experiments. (a) Graphic depiction of the number of genes upregulated (blue) or downregulated (yellow) in EcRi late third instar larvae or prepupae at the times indicated. (b) Graphic depiction of the number of 20E-regulated genes or 20E primary-response genes that are either upregulated (blue) or downregulated (yellow) in third instar larval organ culture. (c) Venn diagram depicting the overlap between all EcR-regulated genes and the combined 20E-regulated genes and 20E primary-response genes. (d) Cluster diagram depicting the temporal expression pattern of the 479 genes in the 20E-final set, divided into those genes that are upregulated by 20E (above) or downregulated by 20E (below). Times are shown in hours relative to puparium formation, and colors are as described in Figure 1b.