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Table 3 ESTs from adult cDNAs with predicted amino-terminal signal peptides and with homologs in C. elegans

From: Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensisand the evolution of secreted proteins in parasites

Cluster Score P Conventional cDNAs Oligo-capped cDNAs Wormpep ID SignalP criteria SignalP scores Signal in C. elegans? Description of C. elegans gene
        C-p Amino acids SP-p SP?   
(a) Signal peptides predicted in both N. brasiliensis and C. elegans
NBC00012 86 6e-18 4 0 CE20223 YYYYS 0.533 16 1.000 Y Y Unknown (similar to NBC00237)
NBC00031 80 3e-16 2 2 CE17924 YYYYS 0.932 18 0.999 Y Y Unknown
NBC00237 84 5e-17 1 2 CE20223 YYYYS 0.671 19 1.000 Y Y Unknown (similar to NBC00012)
NBC00258 145 1e-35 1 0 CE00133 YYYYS 0.524 19 0.999 Y Y FAR-1 fatty acid/retinol-binding protein
NBC00266 129 6e-31 1 0 CE19630 YYYYS 0.662 20 1.000 Y Y Unknown
NBC00314 147 3e-36 1 1 CE03639 YYYYS 0.708 19 0.987 Y Y Transthyretin-like family
NBC00327 94 2e-20 1 0 CE00906 YYYYS 0.542 25 0.998 Y Y Unknown
NBC00336 138 2e-33 1 0 CE23545 YYYYS 0.903 17 1.000 Y Y Unknown
NBC00354 91 4e-21 4 0 CE16530 YYYYS 0.511 17 0.943 Y Y Unknown
NBC00472 215 8e-57 1 0 CE04886 YYYYS 0.319 15 0.999 Y Y Signal sequence receptor
NBC00487 55 7e-09 1 0 CE05972 YYYYS 0.979 21 0.988 Y Y Unknown
NBC00495 51 3e-07 1 1 CE13171 YYYYS 0.566 19 0.999 Y Y Transthyretin-like family
NBC00502 176 3e-45 1 0 CE32298 YYYYS 0.634 20 1.000 Y Y Ectonucleotide pyrophosphatase/phosphodiesterase
NBC00592 80 1e-15 0 3 CE17924 YYYYS 0.920 16 1.000 Y Y Unknown
NBC00606 81 4e-16 0 2 CE02454 YYYYS 0.399 20 1.000 Y Y Similar to O. volvulus hypodermal antigen Ov-17
NBC00615 207 3e-54 0 1 CE04533 YYYYS 0.995 18 1.000 Y Y LBP-1 fatty acid-binding protein
NBC00616 61 3e-10 0 1 CE20257 YYYYS 0.754 19 0.993 Y Y Unknown
NBC00633 153 4e-38 0 1 CE03639 YYYYS 0.450 17 1.000 Y Y Transthyretin-like family
NBC00641 145 1e -35 0 1 CE33289 YYYYS 0.219 19 0.930 Y Y Unknown
NBC00643 102 2e-22 0 2 CE27850 YYYYS 0.961 17 0.999 Y Y Unknown
NBC00706 50 9e-07 0 1 CE06014 YYYYS 0.466 20 1.000 Y Y Unknown
NBC00720 12 3e-30 0 1 CE16958 YYYYS 0.967 19 0.998 Y Y NLP-13 neuropeptide
NBC00742 60 3e-10 0 1 CE16731 YYYYS 0.880 21 0.993 Y Y Unknown
NBC00748 50 4e-07 0 1 CE02932 YYYYS 0.804 17 0.998 Y Y Transthyretin-like family
NBC00767 79 7e-16 0 1 CE31662 YYYYS 0.559 17 1.000 Y Y Unknown
(b) Signal peptides predicted in N. brasiliensis but not C. elegans
NBC00028 104 1e-23 1 1 CE00431 YYYYS 0.731 18 0.999 Y N Globin
NBC00124 128 8e-31 1 1 CE00431 YYYYS 0.731 18 0.999 Y N Globin
NBC00144 195 7e-51 1 0 CE29663 YYNYS 0.866 19 0.963 Y N Transport-secretion protein
NBC00197 143 8e-35 3 6 CE00431 YYYYS 0.557 16 1.000 Y N Globin
NBC00272 144 2e-35 1 0 CE32475 YYNYS 0.262 22 0.513 Y N Unknown
NBC00328 147 4e-36 3 4 CE00431 YYYYS 0.523 17 0.999 Y N Globin
NBC00581 122 7e-29 0 1 CE00431 YYYYS 0.404 21 0.998 Y N Globin
NBC00601 93 5e-20 0 1 CE30218 YYYYS 0.535 34 0.944 Y N Unknown
NBC00607 159 4e-40 0 1 CE29597 YYNYS 0.529 18 0.786 Y N Unknown
  1. Entries in table do not match numbers in Figure 2, which includes predicted signal anchors. SignalP criteria are C-score (raw cleavage site score); S-score (signal peptide score); Y-score (combined cleavage site score); mean S score; and assignation as signal peptide (S as in all entries above; otherwise A for signal anchor or N for neither). SignalP scores are as follows: C-p: probability of predicted cleavage site being correct; amino acids: length of predicted signal peptide in amino acids; SP-p: probability of existence of signal peptide; SP?: overall prediction for signal peptide. Note that NBC00028 is almost identical to the cuticular globin of N. brasiliensis (P51536), and NBC00197 and NBC00328 are closely related, whereas NBC0124 and NBC00581 are more similar to, but not identical to, the body-wall form of globin (P51535).