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Table 2 ESTs from adult cDNAs with known homologs, classified by function

From: Signal sequence analysis of expressed sequence tags from the nematode Nippostrongylus brasiliensisand the evolution of secreted proteins in parasites

Cluster number

Conventional cDNAs

Oligo-capped cDNAs

P

Accession

Description

Proteases/proteosome/ubiquitin

NBC00018

2

0

1e-33

S66528

26S proteinase regulatory complex, non-ATPase chain (Drosophila melanogaster)

NBC00030

2

0

8e-56

U41556

Cysteine protease CPR-6 (Caenorhabditis elegans)

NBC00086

1

0

3e-29

A48454

Cathepsin B-like cysteine proteinase (Ostertagia ostertagi)

   

5e-28

D48435

Cysteine proteinase AC-3 (Haemonchus contortus)

NBC00168

1

0

2e-42

NM_065563

Calpain thiol protease (Caenorhabditis elegans)

NBC00198

1

0

7e-60

NM_073736

Cysteine protease (legumain, asparaginyl endopeptidase) (Caenorhabditis elegans)

NBC00204

3

0

2e-32

NM_072733

Protease (aspartic) (Caenorhabditis elegans)

NBC00231

2

0

5e-90

NM_064106

Serine carboxypeptidase (Caenorhabditis elegans)

NBC00307

1

0

2e-32

NM_015277

Ubiquitin-protein ligase NEDD4-like; neural precursor (Homo sapiens)

NBC00311

1

0

5e-31

NM_073736

Cysteine protease (legumain, asparaginyl endopeptidase) (Caenorhabditis elegans)

NBC00352

2

0

6e-31

NM_065253

Ubiquitin (Caenorhabditis elegans)

NBC00348

1

0

2e-83

A48145

Ubiquitin-conjugating enzyme, UBC-2 (Caenorhabditis elegans)

NBC00362

1

0

1e-76

S17521

Multicatalytic endopeptidase complex (proteasome) zeta chain (Caenorhabditis elegans)

NBC00368

1

0

9e-13

LCE_ORYLA

Low choriolytic enzyme precursor (zinc metalloprotease) (Oryzias latipes)

NBC00377

1

0

3e-75

PSA4_CAEEL

Proteasome subunit, alpha type 4, PAS-3 (Caenorhabditis elegans)

NBC00459

2

1

2e-26

NM_072733

Protease (aspartic) (Caenorhabditis elegans)

NBC00469

1

0

7e-17

NM_060215

Zinc metalloprotease (Caenorhabditis elegans)

NBC00509

1

1

4e-71

AL161503

Polyubiquitin, UBQ10 (Arabidopsis thaliana)

NBC00664

0

1

5e-09

NM_074798

Cathepsin-like (cysteine) protease (Caenorhabditis elegans)

NBC00670

0

1

3e-18

S17435

Polyubiquitin 6 (Helianthus annuus)

NBC00772

0

1

4e-24

NM_003352

Sentrin, ubiquitin-like small protein (Gallus gallus)

NBC00783

0

1

2e-89

U41556

Cysteine protease CPR-6 (Caenorhabditis elegans)

NBC00828

0

1

9e-63

NC_003424

Pad1 protein; 26S proteasome subunit (Schizosaccharomyces pombe)

Enzymes (other than proteases)

NBC00045

2

0

2e-92

NM_065870

Fructose-biphosphate aldolase (Caenorhabditis elegans)

NBC00049

1

0

9e-50

NM_070783

Lipase (Caenorhabditis elegans)

NBC00066

2

1

7e-76

NM_074348

Peptidyl-prolyl cis-trans isomerase (Caenorhabditis elegans)

NBC00079

1

0

2e-35

NM_058712

Helicase (Caenorhabditis elegans)

NBC00102

1

0

7e-37

NM_074031

Peroxidase-like (Caenorhabditis elegans)

NBC00139

1

0

8e-29

NM_060074

Hexokinase (Caenorhabditis elegans)

NBC00143

1

0

4e-66

ADHX_MYXGL

Alcohol dehydrogenase class III (Caenorhabditis elegans)

NBC00147

1

0

6e-19

XM_087230

Similar to Uridine phosphorylase (UDRPase) (Homo sapiens)

NBC00157

1

0

3e-13

XM_058660

Similar to Protein tyrosine phosphatase 1E (Homo sapiens)

NBC00173

1

0

5e-72

AJ440747

Protein disulphide isomerase 1 (Ostertagia ostertagi)

NBC00183

1

0

3e-56

T46280

Isocitrate dehydrogenase, NADP+, cytosolic (Homo sapiens)

NBC00189

1

0

1e-21

XM_129069

Similar to Acetyltransferase (GNAT) family (Mus musculus)

NBC00212

1

0

6e-57

NM_016100

N-terminal acetyltransferase complex ard1 subunit (Homo sapiens)

NBC00283

1

0

4e-27

NM_012088

6-phosphogluconolactonase (Homo sapiens)

NBC00285

1

0

2e-47

LDHA_ANGRO

L-lactate dehydrogenase A chain (Anguilla rostrata)

NBC00290

1

0

3e-17

I55976

Dihydrolipoamide S-acetyltransferase (Rattus norvegicus)

NBC00292

1

0

1e-40

NM_006223

Peptidyl-prolyl cis/trans isomerase (Homo sapiens)

NBC00304

1

0

4e-12

NM_073341

Glucose-1-dehydrogenase (Caenorhabditis elegans)

NBC00309

1

0

1e-18

NM_066225

Hydroxymethylglutaryl-coA reductase (Caenorhabditis elegans)

NBC00326

1

0

1e-65

NM_065761

Protein phosphatase 2A (Caenorhabditis elegans)

NBC00337

1

0

2e-60

GMD1_CAEEL

Probable GDP-mannose 4,6 dehydratase 1 (Caenorhabditis elegans)

NBC00353

1

0

2e-56

NM_065537

ATP synthase B chain (Caenorhabditis elegans)

NBC00378

1

0

2e-43

NM_073253

Acetyltransferase (GNAT) family (Caenorhabditis elegans)

NBC00382

1

0

4e-49

NM_063827

Phospholipase A2 (Caenorhabditis elegans)

NBC00389

2

0

1e-48

NM_058626

Phosphotransferase (Caenorhabditis elegans)

NBC00404

1

0

2e-76

NM_064078

Glucosamine-fructose-6-phosphate aminotransferase (Caenorhabditis elegans)

NBC00413

1

0

6e-22

NM_078324

AMP-activated protein kinase (Caenorhabditis elegans)

NBC00427

1

0

2e-20

NC_003423

3-oxoacyl-(acyl-carrier-protein)-synthase (Schizosaccharomyces pombe)

NBC00475

1

0

3e-42

NM_065313

Serine/threonine protein phosphatase (Caenorhabditis elegans)

NBC00483

1

0

4e-25

NM_059984

Phospholipase, similar to ADRAB-b (Caenorhabditis elegans)

NBC00504

1

0

7e-65

AF292096

Protein kinase AIRK2 (Xenopus laevis)

NBC00508

1

2

5e-64

PPCK_HAECO

Phosphoenolpyruvate carboxykinase (Haemonchus contortus)

NBC00528

1

0

5e-66

PPCK_HAECO

Phosphoenolpyruvate carboxykinase (Haemonchus contortus)

NBC00561

0

7

1e-54

NDKB_RAT

Nucleoside diphosphate kinase B (Rattus norvegicus)

NBC00713

0

1

1e-08

XM_140038

Similar to tau-tubulin kinase (Mus musculus)

NBC00729

0

2

4e-21

NM_079041

Flap endonuclease 1 (Drosophila melanogaster)

NBC00743

0

1

3e-64

G3P_BRUMA

Glyceraldehyde 3-phosphate dehydrogenase (Brugia malayi)

NBC00745

0

1

4e-13

NM_068436

Casein kinase (Caenorhabditis elegans)

NBC00689

0

3

2e-17

CLYC_CAEEL

Serine hydroxymethyltransferase MEL-32 (Caenorhabditis elegans)

NBC00696

0

2

2e-15

NM_000414

Hydroxysteroid (17-beta) dehydrogenase 4 (Homo sapiens)

NBC00770

0

1

3e-45

NM_066907

Serine/threonine kinase, casein kinase-like (Caenorhabditis elegans)

NBC00777

0

1

8e-21

OAZ_PRIPA

Ornithine decarboxylase antizyme (Pristionchus pacificus)

NBC00796

0

1

8e-52

XM_125017)

Putative lysophosphatidic acid acyltransferase (Mus musculus)

NBC00802

0

1

4e-49

NM_078623

Enoyl Coenzyme A hydratase, short chain 1 (Rattus norvegicus)

Structural

NBC00056

1

0

4e-58

NM_071024

Actin depolymerizing factor (Caenorhabditis elegans)

NBC00062

1

0

1e-11

NM_006400

Dynactin 2; dynactin complex 50 kD subunit; dynamitin (Homo sapiens)

NBC00078

2

0

0

NM_059538

Calponin (Caenorhabditis elegans)

NBC00097

1

0

1e-42

MLR1_CAEEL

Myosin regulatory light chain 1 (Caenorhabditis elegans)

NBC00142

1

0

2e-76

S53776

Beta-tubulin isotype I (Haemonchus contortus)

NBC00172

2

0

0

NM_073416

Actin (Caenorhabditis elegans)

NBC00224

1

0

2e-40

NM_063850

Troponin C (Caenorhabditis elegans)

NBC00239

4

1

2e-39

NM_077559

Collagen (Caenorhabditis elegans)

NBC00241

2

0

2e-47

NM_069715

Collagen (Caenorhabditis elegans)

   

6e-47

NM_077291

Cuticular collagen (Caenorhabditis elegans)

NBC00246

1

1

3e-19

NM_077087

Troponin I (Caenorhabditis elegans)

NBC00287

2

0

2e-61

MLR1_CAEEL

Myosin regulatory light chain 1 (Caenorhabditis elegans)

NBC00360

1

1

3e-30

NM_145671

Actinfilin (Rattus norvegicus)

NBC00396

1

0

2e-67

MYSP_CAEEL

Paramyosin (Caenorhabditis elegans)

NBC00403

1

0

3e-32

NM_077291

Cuticular collagen (Caenorhabditis elegans)

NBC00418

1

0

6e-27

NM058881

Calponin (Caenorhabditis elegans)

NBC00430

1

0

3e-11

NM_011722

Dynactin 6; p27 dynactin subunit (Mus musculus)

NBC00526

1

0

2e-44

NM_060857

Profilin (Caenorhabditis elegans)

NBC00552

0

1

9e-47

MYSP_CAEEL

Paramyosin (Caenorhabditis elegans)

NBC00569

0

1

1e-23

NM_060369

Alpha crystallin B chain (Caenorhabditis elegans)

NBC00749

0

1

3e-43

NM_060857

Profilin (Caenorhabditis elegans)

Embryo/egg/mating etc

NBC00068

3

0

1e-25

VIT5_CAEEL

Vitellogenin 5 precursor (Caenorhabditis elegans)

NBC00161

1

0

2e-15

VIT5_CAEEL

Vitellogenin 5 precursor (Caenorhabditis elegans)

NBC00397

1

9

7e-61

MS10_CAEEL

Major Sperm Protein 10 (Caenorhabditis elegans)

NBC00523

1

0

4e-69

XM_038960

Similar to preimplantation protein 3 (Homo sapiens)

NBC00585

0

5

2e-30

NM_076467

Vitellogenin (Caenorhabditis elegans)

NBC00611

0

1

1e-25

NM_060189

Placental protein 11 (Caenorhabditis elegans)

Transporters/receptors/lectins and other binding proteins

NBC00027

2

0

9e-17

NM_062882

Lectin, C-type (Caenorhabditis elegans)

   

5e-15

NM_076712

Asialoglycoprotein receptor (C-type lectin) (Caenorhabditis elegans)

NBC00110

1

0

4e-17

NC_001263

Acyl-CoA-binding protein (Deinococcus radiodurans)

NBC00118

1

0

4e-41

T31073

Multidrug resistance P-glycoprotein (Haemonchus contortus)

NBC00128

3

0

1e-92

NM_067381

ADP/ATP carrier protein/translocase (Caenorhabditis elegans)

NBC00167

1

0

2e-12

NM_130415

Lysosomal amino acid transporter 1 (Rattus norvegicus)

NBC00175

1

0

7e-15

A48925

Mannose receptor (C-type lectin), macrophage (Mus musculus)

NBC00319

1

0

8e-15

NXT2_HUMAN

NTF2-related export protein 2 (p15-2 protein) (Homo sapiens)

NBC00324

2

0

7e-15

AJ243873

Galectin (S-type lectin) (Haemonchus contortus)

NBC00340

1

0

2e-61

NM_077246

Galectin (S-type lectin) LEC-10 (Caenorhabditis elegans)

NBC00355

1

0

8e-21

NM_059527

Fatty acid-binding protein LBP-6 (Caenorhabditis elegans)

NBC00363

1

0

6e-48

NM_016208

Vacuolar protein sorting 28 homolog (Homo sapiens)

NBC00583

0

5

4e-35

NM_065836

Low density lipoprotein receptor (Caenorhabditis elegans)

NBC00593

0

2

2e-26

NM_059525

Fatty acid-binding protein LBP-6 (Caenorhabditis elegans)

NBC00752

0

1

3e-08

NM_059071

Acetylcholine receptor UNV-38 (Caenorhabditis elegans)

NBC00766

0

1

7e-44

POR2_MELGA

Voltage-dependent anion-selective channel protein 2 (VDAC-2) (Meleagris gallopavo)

NBC00808

0

1

6e-53

NM_072174

Calreticulin precursor (Caenorhabditis elegans)

NBC00838

0

1

1e-78

NM_063349

T-complex protein, delta subunit (cytosolic chaperonin CCT-4) (Caenorhabditis elegans)

Signaling

NBC00207

1

0

0

RAB2_LYMST

RAS-Related protein RAB-2 (Lymnea stagnalis)

NBC00252

1

0

8e-97

NM_070558

RAS-like GTP-binding protein RhoA (Caenorhabditis elegans)

NBC00297

1

0

4e-17

NM_009106

Rhotekin (Mus musculus)

NBC00312

1

0

4e-46

A35350

Protein kinase C inhibitor (Bos bovis)

NBC00269

1

0

1e-43

NM_058274

RAS-related protein RAB-11 (Caenorhabditis elegans)

NBC00282

1

0

9e-25

NP_741191

A kinase anchor protein 1 (Caenorhabditis elegans)

NBC00395

1

0

2e-29

NM_07328

RAS-like GTP-binding protein (cdc42-like) (Caenorhabditis elegans)

NBC00436

1

0

2e-44

NM_070985

Calmodulin (Caenorhabditis elegans)

NBC00462

1

0

2e-13

SSRP_DROME

Single-strand recognition protein (SSRP) (Chorion-factor 5) (Drosophila melanogaster)

NBC00409

1

0

1e-16

NM_019746

Programmed cell death 5/TFAR19 protein (Mus musculus)

NBC00440

1

0

3e-72

S43599

SNF5 homolog R07E5.3 (Caenorhabditis elegans)

NBC00510

1

0

2e-28

XM_129572

Calcyclin (S100 family) binding protein (Mus musculus)

NBC00629

0

1

1e-20

NM_026297

RAB (RAS oncogene family-like 3) (Mus musculus)

NBC00648

0

1

3e-20

NM_002624

Prefoldin 5 isoform alpha; myc modulator-1; c-myc binding protein (Homo sapiens)

NBC00727

0

1

3e-17

AB091687

TGF-beta induced apotosis protein 3 (Mus musculus)

NBC00768

0

1

3e-18

NM_078471

TGF-beta-1 induced anti-apoptotic factor 1 isoform 1 (Homo sapiens)

NBC00829

0

1

1e-42

A49146

Developmental regulator WNT-4 (Xenopus laevis)

NBC00841

0

1

1e-31

NM_012453

Transducin (beta)-like 2, isoform 1 (Homo sapiens)

DNA-related/transcription/DNA binding/regulation

NBC00024

1

0

1e-37

NM_003752

Eukaryotic translation initiation factor 3, subunit 8 (Homo sapiens)

NBC00048

1

0

1e-28

NM_069150

Glycine-rich RNA-binding protein (Caenorhabditis elegans)

   

5e-21

NM_007007

Cleavage and polyadenylation specific factor 6 (Homo sapiens)

NBC00050

1

0

2e-12

HEXP_LEIMA

DNA-binding protein HEXBP (Hexamer-binding protein) (Leishmania major)

NBC00055

1

1

2e-24

NM_060622

RNA recognition motif (RRM, RBD, or RNP domain) (Caenorhabditis elegans)

NBC00090

2

1

0

NM_066119

Elongation factor 1-alpha (Caenorhabditis elegans)

NBC00099

1

0

2e-30

NM_067248

Splicing factor (Caenorhabditis elegans)

NBC00170

1

0

2e-56

NM_011304

RuvB DNA helicase -like protein 2 (Mus musculus)

NBC00181

1

0

4e-13

NM_001698

AU RNA-binding protein/enoyl-Coenzyme A hydratase (Homo sapiens)

NBC00192

1

0

2e-26

NM_060622

RNA recognition motif (RRM, RBD, or RNP domain) (Caenorhabditis elegans)

NBC00210

1

0

3e-15

NM_018403

Transcription factor (SMIF gene) (Homo sapiens)

NBC00267

1

0

4e-20

T2EB_XENLA

Transcription initiation factor IIE, beta subunit (Xenopus laevis)

NBC00321

1

0

1e-16

NM_033224

Purine-rich element binding protein B (Homo sapiens)

NBC00280

1

0

3e-58

NM_006578

Guanine nucleotide-binding protein, beta-5 subunit (Homo sapiens)

NBC00350

1

0

6e-40

DPOD_DROME

DNA polymerase delta catalytic subunit (Drosophila melanogaster)

NBC00366

2

0

6e-79

NM_066119

Elongation factor 1-alpha (Caenorhabditis elegans)

NBC00370

1

0

1e-17

NM_031992

Eukaryotic translation initiation factor 4H, isoform 2 (Homo sapiens)

NBC00374

1

2

2e-53

NM_070415

Elongation factor 1-beta/delta chain (Caenorhabditis elegans)

NBC00480

1

0

3e-21

NM_061014

Regulator of chromosome condensation, RCC1 (Caenorhabditis elegans)

NBC00543

0

2

5e-23

NM_065536

Zinc finger, C3HC4 type (RING finger) (Caenorhabditis elegans)

NBC00577

0

7

2e-31

NP_872244

Translation elongation factor EFT-4 (Caenorhabditis elegans)

NBC00600

0

1

3e-74

NM_063406

Initiation factor 5A (Caenorhabditis elegans)

NBC00630

0

1

9e-39

SFR4_MOUSE

Splicing factor, arginine/serine-rich 4 (Mus musculus)

NBC00764

0

1

4e-16

XM_132357

Similar to Translation Initiation factor EIF-2B alpha (Mus musculus)

NBC00776

0

1

6e-27

SN2L_CAEEL

Potential global transcription activator SNF2L (Caenorhabditis elegans)

NBC00791

0

1

5e-38

NM_001207

Basic transcription factor 3 (Homo sapiens)

NBC00816

0

1

2e-24

S3B2_HUMAN

Splicing factor 3B subunit 2 (Spliceosome associated protein 145) (Homo sapiens)

Other homologs of interest

NBC00025

1

0

3e-16

AF352714

HC40 putative secretory protein precursor (ASP homolog) (Haemonchus contortus)

NBC00065

1

0

6e-20

AA063577

Secreted protein 5 precursor (ASP homolog) (Ancylostoma caninum)

NBC00095

1

0

8e-59

GLB2_NIPBR

Myoglobin (body wall isoform globin) (Nippostrongylus brasiliensis)

NBC00103

1

0

9e-12

DIM1_CAEEL

Protein dim-1 (2D-page protein spot 8) (Caenorhabditis elegans)

NBC00029

1

0

5e-17

NM_001545

Immature colon carcinoma transcript 1 (Homo sapiens)

NBC00141

1

0

2e-35

NM_018984

Slingshot 1 (Homo sapiens)

NBC00160

1

0

5e-12

NM_053810

Synaptosomal-associated protein, 29kD (Rattus norvegicus)

NBC00199

1

0

9e-39

AF278538

Nucleosome assembly protein 1 (Xenopus laevis)

NBC00256

2

0

2e-09

NM_075227

Transthyretin-like family (Caenorhabditis elegans)

NBC00293

1

0

7e-08

NC_003424

F-box protein (Schizosaccharomyces pombe)

NBC00399

1

0

2e-22

NM_076443

Calumenin, calcium-binding protein (Caenorhabditis elegans)

NBC00429

1

0

4e-14

XM_122362

Chromobox homolog 2 (Drosophila Pc class) (Mus musculus)

NBC00491

1

0

3e-21

NM_076885

Thrombospondin (Caenorhabditis elegans)

NBC00518

1

0

3e-73

T37461

Mago nashi-like protein (Caenorhabditis elegans)

NBC00544

0

1

2e-45

NM_061213

Alpha-2-macroglobulin family (Caenorhabditis elegans)

NBC00560

0

1

1e-35

NM_021305

SEC61, alpha subunit 2 (Saccharomyces cerevisiae)

NBC00705

0

1

3e-31

DVA1_DICVI

DVA-1 nematode polyprotein allergen precursor (NPA) (Dictyocaulus viviparus)

   

2e-12

ABA1_ASCSU

ABA-1 nematode polyprotein allergen precursor (Body fluid allergen-1) (Ascaris suum)

NBC00753

0

1

4e-10

AF089728

Ancylostoma-secreted protein 2 precursor, ASP-2 (Ancylostoma caninum)

NBC00755

0

1

2e-40

TCPB_CAEEL

T-complex protein 1, beta subunit (CCT-beta) (Caenorhabditis elegans)

NBC00757

0

1

2e-68

1432_SCHMA

14-3-3 Protein homolog 2 (14-3-3-2) (Schistosoma mansoni)

NBC00803

0

1

3e-09

ASP_ANCCA

Ancylostoma secreted protein (ASP-1) precursor (Ancylostoma caninum)

   

3e-09

AF079521

Ancylostoma-secreted protein 1 precursor (ASP-1 homolog) (Necator americanus)

NBC00827

0

1

3e-14

NM_070108

Testis-specific protein TPX-1 like (ASP homolog) (Caenorhabditis elegans)

  1. The table gives, for each numbered cluster, the highest homolog with a functional description where available; in a number of cases a C. elegans homolog exists with a higher similarity, but has no description. Similarities to entries described as 'hypothetical proteins' are excluded, as are heat-shock proteins, cytochromes, mitochondrial and ribosomal products. Where C. elegans protein description is ambiguous (for example, protease, lectin), further descriptors added manually are italicized. Different clusters may derive from a single gene if sequences are non-overlapping; for example, NBC00198 and NBC00311 align to different segments of the C. elegans protease gene NM_073736. This table does not include N. brasiliensis gene products discovered previously and/or reported by other laboratories. All entries for this species are aggregated on the NEMBASE website.