Figure 5
Number of samples yielding measurable data for each antibody. RCA (gray bars) was compared to either direct labeling or indirect detection (dark bars) in the measurement of 24 serum samples in duplicate. For each antibody, the number of samples producing measurements above the detection threshold (as defined in Materials and methods) was summed and averaged over the duplicate experiment sets (see Table 1 for antibody IDs and summary of correlations between replicate experiment sets). (a) RCA compared to direct labeling on hydrogels using samples from liver cancer patients and controls. (b) RCA compared to indirect detection on hydrogels using samples from liver cancer patients and controls. (c) RCA compared to indirect detection on nitrocellulose using samples from liver cancer patients and controls.