Predictions and ARS assay results compared to probable origin locations. (a) Shown are predictions in the top 100 that did not match the evaluation set along with their ARS activities. Likely origins in the evaluation set are in blue (ORC/MCM), and Oriscan predictions are in black. The width of the bars is not to scale. Vertical gray lines drawn through predictions show whether there is overlap with an evaluation set member. ARS assay results are scored on a scale of 0 to 3 for origin strength; 0 indicates inactivity, 1 indicates weak activity, and 2 and 3 indicate increasingly strong activity. Chromosomes are identified in Roman numerals at the top left of each plot, and positions in kb are given beneath the axis. Each prediction assayed is given a lowercase letter in red for reference in the text. For legibility, ARS assay results are offset for the pair of closely spaced predictions on chromosome IX. (b) All predictions and ARS assay results on chromosome XV. Plotting conventions are as in (a), except that origins which were tested after mutation of the ACS (f, j, and m) have a number indicating the ARS activity of the mutant in red under the original number. There are two very closely spaced predictions at 715 kb (g); neither was active, and this is denoted with a single 0.