Figure 1

Process flow and in-process quality control for manufacturing the Drosophila FlyGEM. (a) The process flow for generation of PCR product for arraying and in-process quality-control measures (QC) are represented. (b) Barcode primers are located at unique positions in each plate (red wells in plate cartoon). (c) A typical agarose gel following electrophoresis of amplicons. The barcode amplicons migrate more slowly and allow for tracking after PCR (red asterisk). (d) 96-well plates of purified PCR product were collapsed into ten 1,536-well plates for printing. An individual plate barcode position (red) and all other barcodes (green) are shown. Post-hybridization QCs included (e) oligo hybridization to the barcoding elements and (f) syto-staining. Note the unprinted area available for adding new elements to the platform.