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Table 3 Bacterial strains

From: In silico identification and experimental validation of PmrAB targets in Salmonella typhimuriumby regulatory motif detection

Strain or plasmid

Relevant genotype

Reference or source

Salmonella

ATCC 14028s

Wild type

ATCC

 

JSG421

pmrA::Tn10d

[21] kind gift of J.S. Gunn

 

LB5010

metA22 metE551 ilv-452 leu-3121 trpΔ2 xyl-404 galE856 hsdLT6 hsdSA29 hsdSB121 rpsL120

[67]

E. coli

DH5α

Fφ80ΔlacZM15 Δ(lacZYAargF)U169 deoP recA1 endA1 hsdR17 (rk-mk-)

Gibco BRL

Plasmids

pCRII-TOPO

Cloning vector, AmpR

Invitrogen

 

pFPV25

ColE1 mob bla promoterless gfpmut3, AmpR

[40] kind gift of R. Valdivia and S. Falkow

 

pFPV25.1

Constitutive rpsM promoter in pFPV25, AmpR

[40] kind gift of R. Valdivia and S. Falkow

 

pCMPG5611

pFPV25 with yibD promoter

This work

 

pCMPG5612

pFPV25 with ybjG promoter

This work

 

pCMPG5613

pFPV25 with STM1269 promoter

This work

 

pCMPG5614

pFPV25 with yjdB promoter

This work

 

pCMPG5621

pFPV25 with sseJ promoter

This work

 

pCMPG5615

pCMPG5611 with point mutated putative PmrA motif C→A

This work

 

pCMPG5616

pCMPG5611 with point mutated putative PmrA motif T→G

This work

 

pCMPG5617

pCMPG5611 with point mutated putative PmrA motif T→G

This work

 

pCMPG5618

pCMPG5611 with point mutated putative PmrA motif A→C

This work

 

pCMPG5619

pCMPG5611 with point mutated putative PmrA motif A→C

This work

 

pCMPG5620

pCMPG5611 with point mutated putative PmrA motif T→C

This work