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Table 3 Bacterial strains

From: In silico identification and experimental validation of PmrAB targets in Salmonella typhimuriumby regulatory motif detection

Strain or plasmid Relevant genotype Reference or source
Salmonella ATCC 14028s Wild type ATCC
  JSG421 pmrA::Tn10d [21] kind gift of J.S. Gunn
  LB5010 metA22 metE551 ilv-452 leu-3121 trpΔ2 xyl-404 galE856 hsdLT6 hsdSA29 hsdSB121 rpsL120 [67]
E. coli DH5α Fφ80ΔlacZM15 Δ(lacZYAargF)U169 deoP recA1 endA1 hsdR17 (rk-mk-) Gibco BRL
Plasmids pCRII-TOPO Cloning vector, AmpR Invitrogen
  pFPV25 ColE1 mob bla promoterless gfpmut3, AmpR [40] kind gift of R. Valdivia and S. Falkow
  pFPV25.1 Constitutive rpsM promoter in pFPV25, AmpR [40] kind gift of R. Valdivia and S. Falkow
  pCMPG5611 pFPV25 with yibD promoter This work
  pCMPG5612 pFPV25 with ybjG promoter This work
  pCMPG5613 pFPV25 with STM1269 promoter This work
  pCMPG5614 pFPV25 with yjdB promoter This work
  pCMPG5621 pFPV25 with sseJ promoter This work
  pCMPG5615 pCMPG5611 with point mutated putative PmrA motif C→A This work
  pCMPG5616 pCMPG5611 with point mutated putative PmrA motif T→G This work
  pCMPG5617 pCMPG5611 with point mutated putative PmrA motif T→G This work
  pCMPG5618 pCMPG5611 with point mutated putative PmrA motif A→C This work
  pCMPG5619 pCMPG5611 with point mutated putative PmrA motif A→C This work
  pCMPG5620 pCMPG5611 with point mutated putative PmrA motif T→C This work