Skip to main content

Table 2 Genes affiliated to sulfur metabolism

From: Integrating phenotypic and expression profiles to map arsenic-response networks

Gene Open reading frame AsIII Cd2+ H2O2 Enzyme
   Fold change  
Sulfate assimilation      
MET3 YJR010W 5.0-20.0 10.8 -1.0-3.0 ATP sulfurylase
MET14 YKL001C 2.0-14.0 9.6 -2.0-3.0 APS kinase
MET16 YPR167C 2.0-12.0 9.1 NC PAPS reductase
MET22 (0.5 h, 1 mM AsIII) YOL064C 3.3 2.5 NC Diphosphonucleoside phosphohydrolase
MET10 YFR030W 3.0-5.0 5.2 NC Sulfite reductase alpha
MET5/ECM17 YJR137C 2.0-5.0 4.5 -2.0-4.0 Sulfite reductase beta
MET1/20 YKR069W NC 6 NC Uroporphyrinogen III methylase
MET8 YBR213W NC 7 -2.0-4.0 Siroheme synthase
Sulfide incorporation and transulfuration pathways      
MET2 YNL277W 4.0-3.0 4.6 NC Homoserine transacetylase
MET25/17 YLR303W NC 4.8 NC O-Acetylhomoserine sulfhydrylase
STR4/CYS4 YGR155W 2.0-2.7 2.5 -2.0-3.0 Cystathionine B-synthase
STR1/CYS3 (4 h, 100 μM AsIII) YAL012W -3.5 13.4 NC Cystathionine-lyase
STR3 YGL184C 2.2-3.9 13.5 2.0-4.0 Cystathionine G-synthase
YFR055W YFR055W -9.0-5.0 -1.1 NC Cystathionine-lyase
Methionine and AdoMet biosynthesis      
MET6 YER091C 1.0-3.5 NC -2.0-5.0 N5-Methyltetrahydrofolate homocysteine transferase
MET7 YOR241W NC -1.6 NC Tetrahydrofolyl polyglutamate synthase
MET13 YGL125W NC NC -1.0-3.0 Methylene tetrahydrofolate reductase
SAM1 (4 h, 100 μM AsIII) YLR180W 2.5 NC -2.0-9.0 AdoMet synthetase
SAM2 (4 h, 100 μM AsIII) YDR502C 3.8 NC -2.0-4.0 AdoMet synthetase
MHT1 YLL062C 5.0-2.8 10.6 NC S-methylmethionine: homocysteine S-methyltransferase
Sulfur compound uptake      
SUL1 YBR294W 5.4-2.4 20 NC Sulfate transporter
SUL2 YLR092W 2.5-2.8 3 NC Sulfate transporter
MUP1 (2 h and 4 h, 100 μM AsIII) YGR055W 5.0-14.0 2 -1.0-4.0 Methionine permease, high affinity
MUP3 YHL036W 8.0-7.0 7   Methionine permease, low affinity
Regulatory genes      
MET4 (2 h, 100 μM AsIII) YNL103W 2 1.5 NC bZIP
MET28 YIR017C NC 5 NC bZIP
CBF1 YJR060W NC -2 NC bHLH
MET30 YIL046W 5.0-1.6 7 NC WD40 repeats F box
MET31 YPL039W NC 1 -1.0-7.0 Zinc finger
MET32 YDR253C 6.0-3.6 14 -1.0-4.0 Zinc finger
  1. Arsenic treatment stimulates a sulfur response in yeast. Gene expression data comparisons between arsenic, cadmium, and H2O2-treated Saccharomyces cerevisiae reveal arsenic and cadmium mediated sulfur responses, but none with hydrogen peroxide. AsIII column, 2 h, 100 μM and 0.5 h, 1 mM (combined biological replicates), unless noted; cadmium column, 1 h, 1 mM [63]; H2O2 column, 10, 20, 40, 60, 120 min, 400 μM [62]. Numbers in ordinary typeface denote induction; (-) and italicized numbers denote repression; NC, no change.