Table 2 Genes affiliated to sulfur metabolism
From: Integrating phenotypic and expression profiles to map arsenic-response networks
Gene | Open reading frame | AsIII | Cd2+ | H2O2 | Enzyme |
|---|---|---|---|---|---|
| Â | Â | Fold change | Â | ||
Sulfate assimilation | Â | Â | Â | Â | Â |
MET3 | YJR010W | 5.0-20.0 | 10.8 | -1.0-3.0 | ATP sulfurylase |
MET14 | YKL001C | 2.0-14.0 | 9.6 | -2.0-3.0 | APS kinase |
MET16 | YPR167C | 2.0-12.0 | 9.1 | NC | PAPS reductase |
MET22 (0.5 h, 1 mM AsIII) | YOL064C | 3.3 | 2.5 | NC | Diphosphonucleoside phosphohydrolase |
MET10 | YFR030W | 3.0-5.0 | 5.2 | NC | Sulfite reductase alpha |
MET5/ECM17 | YJR137C | 2.0-5.0 | 4.5 | -2.0-4.0 | Sulfite reductase beta |
MET1/20 | YKR069W | NC | 6 | NC | Uroporphyrinogen III methylase |
MET8 | YBR213W | NC | 7 | -2.0-4.0 | Siroheme synthase |
Sulfide incorporation and transulfuration pathways | Â | Â | Â | Â | Â |
MET2 | YNL277W | 4.0-3.0 | 4.6 | NC | Homoserine transacetylase |
MET25/17 | YLR303W | NC | 4.8 | NC | O-Acetylhomoserine sulfhydrylase |
STR4/CYS4 | YGR155W | 2.0-2.7 | 2.5 | -2.0-3.0 | Cystathionine B-synthase |
STR1/CYS3 (4 h, 100 μM AsIII) | YAL012W | -3.5 | 13.4 | NC | Cystathionine-lyase |
STR3 | YGL184C | 2.2-3.9 | 13.5 | 2.0-4.0 | Cystathionine G-synthase |
YFR055W | YFR055W | -9.0-5.0 | -1.1 | NC | Cystathionine-lyase |
Methionine and AdoMet biosynthesis | Â | Â | Â | Â | Â |
MET6 | YER091C | 1.0-3.5 | NC | -2.0-5.0 | N5-Methyltetrahydrofolate homocysteine transferase |
MET7 | YOR241W | NC | -1.6 | NC | Tetrahydrofolyl polyglutamate synthase |
MET13 | YGL125W | NC | NC | -1.0-3.0 | Methylene tetrahydrofolate reductase |
SAM1 (4 h, 100 μM AsIII) | YLR180W | 2.5 | NC | -2.0-9.0 | AdoMet synthetase |
SAM2 (4 h, 100 μM AsIII) | YDR502C | 3.8 | NC | -2.0-4.0 | AdoMet synthetase |
MHT1 | YLL062C | 5.0-2.8 | 10.6 | NC | S-methylmethionine: homocysteine S-methyltransferase |
Sulfur compound uptake | Â | Â | Â | Â | Â |
SUL1 | YBR294W | 5.4-2.4 | 20 | NC | Sulfate transporter |
SUL2 | YLR092W | 2.5-2.8 | 3 | NC | Sulfate transporter |
MUP1 (2 h and 4 h, 100 μM AsIII) | YGR055W | 5.0-14.0 | 2 | -1.0-4.0 | Methionine permease, high affinity |
MUP3 | YHL036W | 8.0-7.0 | 7 | Â | Methionine permease, low affinity |
Regulatory genes | Â | Â | Â | Â | Â |
MET4 (2 h, 100 μM AsIII) | YNL103W | 2 | 1.5 | NC | bZIP |
MET28 | YIR017C | NC | 5 | NC | bZIP |
CBF1 | YJR060W | NC | -2 | NC | bHLH |
MET30 | YIL046W | 5.0-1.6 | 7 | NC | WD40 repeats F box |
MET31 | YPL039W | NC | 1 | -1.0-7.0 | Zinc finger |
MET32 | YDR253C | 6.0-3.6 | 14 | -1.0-4.0 | Zinc finger |