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Table 3 Replication timing of artificial chromosomes

From: Assembly and characterization of heterochromatin and euchromatin on human artificial chromosomes

  Artificial chromosomes Replication timing
    Early: 0-2 h S Mid: 2-4 h S Mid: 4-6 h S Late: 6-8 h S
Line Chromatin composition* Replication timing L U L U L U L U
17-E29 Euchromatin Early/mid 15 10 4 15 1 29 0 26
17-D34 Euchromatin Early/mid 16 11 26 13 0 31 0 32
17-B12 Euchromatin/heterochromatin Mid 2 24 14 15 9 19 0 30
17-C20 Euchromatin/heterochromatin Mid 0 56 20 57 0 57 0 57
Controls           
17 cen Heterochromatin Mid 5 182 27 166 46 209 7 236
Yq Heterochromatin Mid/late 0 138 1 166 70 116 118 81
  1. The number of either labeled (L) or unlabeled (U) artificial chromosomes in lines 17-E29, 17-D34, 17-B12 or 17-C20 or host control 17 centromere regions (17 cen) or Y long arm sequences (Yq) following BrdU detection at 2 h intervals in S phase is indicated in columns early, mid or late S phase. *Chromatin composition of artificial chromosomes in the four lines indicated or control host 17 centromere or Yq regions (see Table 2). Euchromatin: euchromatin present; heterochromatin depleted. Euchromatin/heterochromatin: both euchromatin and heterochromatin present. Heterochromatin: predominantly heterochromatin; euchromatin depleted. Predominant phase in S phase during which replication occurs: early/mid: first half (0-4 h) of S phase; mid S phase (2-6 h into S phase); mid to late S (4-8 h into S phase). Pooled data from all experiments were used to generate the numbers for the controls.