Figure 1From: Transcriptome analysis of haploid male gametophyte development in ArabidopsisSpore isolation and numbers of genes expressed throughout Arabidopsis male gametophyte development. (a) Purity of isolated spores in each developmental stage determined by microscopy: UNM, microspores; BCP, bicellular pollen; TCP, tricellular pollen; MPG, mature pollen. (b-e) DAPI-stained populations of developing spores: (b) microspores; (c) bicellular pollen; (d) tricellular pollen; and (e) mature pollen. (f) Total number of genes expressed in developing pollen and their distribution among three relative abundance classes. Gene-abundance classes were defined as follows: high (up to 10-fold less than the maximum signal), medium (10- to 100-fold less) and low (more than 100-fold less).Back to article page