A reverse genetic screen in Drosophila using a deletion-inducing mutagen

Table 1 Mutation rates induced by HMPA treatment as assessed from white^- mutations at the transgene insertions on chromosomes 2 and 3

white⁺ loci	Brood	Mutation rate (× 10^-4)	Mosaicism at white⁺ loci
Both loci	2A + 2B and 3A	2.25
On chromosome 2	2A + 2B and 3A	2.68	1/14
On chromosome 3	2A + 2B and 3A	2.0	1/10
Both loci	2A + 2B	2.35
Both loci	3A	2.33

Numbers are based on 19 mutations recovered after screening of brood 1 has ceased. Two out of a total of 24 white^- mutants (five mutants were recovered while brood 1 was screened) were mosaic for the mutation. Mutations on chromosome 2 were slightly more common than mutations on chromosome 3; brood-2- and brood-3-derived mutations were equally frequent.

ISSN: 1474-760X