Development of a method for screening short-lived proteins using green fluorescent protein

Table 1 The estimated half-lives of 22 labile proteins

Clone	Accession number	Gene description	Stability	Estimated half-life (h)
2	BC005843	Similar to SH3-containing protein	Short-lived	2
3	AF176555	A-kinase anchoring protein	Short-lived	3
4	AF209502	Calpain	Short-lived	1
5	U87277	Splicing factor SRp30c	Short-lived	0.5
9	BC000804	Adaptor-related protein complex	Short-lived	2
10	BC011384	ATP synthase, H⁺ transporting	Short-lived	2
12	BC005380	Apolipoprotein A-I	Short-lived	2
14	BC007513	H19, imprinted maternally expressed	Short-lived	2
18	AP000014	Function unknown	Short-lived	2
19	M69013	Guanine-nucleotide-binding regulatory protein	Short-lived	0.5
21	BC003128	CGI-89 protein	Short-lived	2
22	AL109795	Function unknown	Short-lived	2
23	X89399	Ins(1,3,4,5)P₄-binding protein	Short-lived	2
25	BC057397	Heat shock 70 kDa protein 1A	Short-lived	2
26	NM_015416	Cervical cancer 1 proto-oncogene protein p40	Short-lived	2
27	AF248272	Gag-Pro-Pol precursor protein gene.	Short-lived	3
28	X07868	Insulin-like growth factors II	Short-lived	2
29	NM_000062	Serine (or cysteine) proteinase inhibitor	Short-lived	2
30	AF068706	Gamma2-adaptin (G2AD)	Short-lived	2
32	AK054590	Function unknown	Short-lived	3
33	BC000404	Thyroid hormone receptor interactors 13	Short-lived	2

The clones were recovered as described in the text and their half-lives were estimated by FACS-based analysis of CHX chase kinetics. All 22 clones were partially sequenced and BLAST analysis performed to identify similar protein sequences in the NCBI public database.

ISSN: 1474-760X