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Table 1 The estimated half-lives of 22 labile proteins

From: Development of a method for screening short-lived proteins using green fluorescent protein

Clone Accession number Gene description Stability Estimated half-life (h)
2 BC005843 Similar to SH3-containing protein Short-lived 2
3 AF176555 A-kinase anchoring protein Short-lived 3
4 AF209502 Calpain Short-lived 1
5 U87277 Splicing factor SRp30c Short-lived 0.5
9 BC000804 Adaptor-related protein complex Short-lived 2
10 BC011384 ATP synthase, H+ transporting Short-lived 2
12 BC005380 Apolipoprotein A-I Short-lived 2
14 BC007513 H19, imprinted maternally expressed Short-lived 2
18 AP000014 Function unknown Short-lived 2
19 M69013 Guanine-nucleotide-binding regulatory protein Short-lived 0.5
21 BC003128 CGI-89 protein Short-lived 2
22 AL109795 Function unknown Short-lived 2
23 X89399 Ins(1,3,4,5)P4-binding protein Short-lived 2
25 BC057397 Heat shock 70 kDa protein 1A Short-lived 2
26 NM_015416 Cervical cancer 1 proto-oncogene protein p40 Short-lived 2
27 AF248272 Gag-Pro-Pol precursor protein gene. Short-lived 3
28 X07868 Insulin-like growth factors II Short-lived 2
29 NM_000062 Serine (or cysteine) proteinase inhibitor Short-lived 2
30 AF068706 Gamma2-adaptin (G2AD) Short-lived 2
32 AK054590 Function unknown Short-lived 3
33 BC000404 Thyroid hormone receptor interactors 13 Short-lived 2
  1. The clones were recovered as described in the text and their half-lives were estimated by FACS-based analysis of CHX chase kinetics. All 22 clones were partially sequenced and BLAST analysis performed to identify similar protein sequences in the NCBI public database.