Figure 2

Developmental profiling. (a) Two-color hybridization (green: adult stage; red: 4-8 h old embryo) on the Heidelberg FlyArray directly showing the expression of genes unique to the Heidelberg Prediction (see lower part, spots within the green rectangle). (b) Correspondence cluster analysis of the developmental profiling. Samples from nine different stages of the Drosophila life-cycle were hybridized to the Heidelberg FlyArray. Each experiment was performed at least in triplicate, including a dye reversal to avoid bias. In the resulting plot, each hybridization of an individual developmental stage is depicted as a colored square for each replicate present on the slide. They all form distinct clusters (except for the larval stage), indicating the degree of reproducibility and specificity between them. As a consequence of the normalization process, only the median of all control hybridizations (0-4 h) is shown in the diagram as a single red square. Genes are shown as grey dots if they exhibited significant differential transcription levels. The distance between dots is low when their expression profiles show similar shape, independent of their absolute values. Colored guiding lines are displayed that correspond to the transcription profiles of virtual genes that would exhibit a signal in one condition only.