Relationship between ORF length and transcript length. Measured transcript length in nucleotides (nts) is plotted against ORF length in base pairs (bps). Green squares and red triangles indicate ORFs whose length is greater than their transcript length and transcripts greater than their theoretical maximum length, respectively. Parentheses indicate how many spots of each type are plotted. The black line is the linear least-squares fit to the blue 'Good' ORF circles. It has the parameters y = 1.0031x + 311.88 (R2 = 0.93).