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Table 4 Summary statistics of DNA polymorphisms analyzed in the study

From: Ancient origin and evolution of the Indian wolf: evidence from mitochondrial DNA typing of wolves from Trans-Himalayan region and Pennisular India

  Mitochondrial domain used for taxonomic comparisions
Sequence Parameters Complete Cyto-B gene Partial Cyto-B gene 16S rRNA gene D-loop (CR-I) 233 bp D-loop (CR-I) 545 bp D-loop (CR-I) 651 bp D-loop (CR-I) 675 bp D-loop Region-II
Sequences compared 5 35 27 80 50 33 24 11
Sequence size compared (bp) 1140 332 554 233 545 651 675 294
Sequence size without gaps/'N' (bp) 1132 280 554 210 505 608 656 252
Polymorphic nucleotides 232 77 32 35 72 73 75 22
Singleton polymorphic nucleotides 178 34 . 14 33 39 44 16
Informative nucleotides 54 43 32 21 39 34 31 6
Base composition (average)         
A : 28.9% 28.2% 26.7% 30.5% 26.9% 27.3% 27.2% 33.6%
C : 27.6% 23.0% 20.3% 24.0% 27.1% 26.5% 25.6% 21.4%
G : 14.2% 16.6% 21.7% 12.3% 14.9% 15.3% 16.3% 11.3%
T : 29.4% 32.2% 31.3% 33.2% 31.1% 30.9% 30.9% 33.7%
Observed Transition/transversion ratios (over all sites/sequences/pair-wise comparisions)
Average 4.15 8.70 11.65 19.57 9.89 7.60 6.96 14.20
Mimimum 2.80 0.00 5.70 0.00 0.00 0.00 0.00 0.00
Maximum 18.75 26.00 11.00 15.00 23.00 24.00 25.00 12.00
Observed substitution statistics
   Over all sites/sequences/pair-wise comparisions
   No. of haplotypes 5 18 6 36 39 28   10
   Number of Average substitutions 106.0+66.0 12.35+9.57 7.21+0.93 4.97+0.15 9.65+0.83 11.15+1.7   5.53+1.5
   Nucleotide diversity (%) 9.39+4.87 4.37+1.15 1.30+0.50 2.37+0.04 1.91+0.04 1.83+0.03   2.19+0.03
   Ks (%) 57.2+48.6 19.2+16.5       
   Ka (%) 1.49+0.79 0.77+0.80       
   HW vs. GW and wolf/dog popun from elsewhere
   Number of Average substitutions     14.1+1.6 20.7+1.9 21.3+1.9   7.1+1.6
   Miminum-maximum substitutions     11-16 17-24 18-25   5-9
   Median (Mode) substitutions     14 (16) 20 (19) 21 (21)   7 (7)
   GW vs. HW and wolf/dog popun from elsewhere
   Number of Average substitutions     13.4+1.3 17.3+1.6 18.0+2.0   5.1+1.6
   Miminum-maximum substitutions     10-17 14-21 14-21   4-9
   Median (Mode) substitutions     13 (13) 17 (17) 18 (19)   5 (4)
   Between different wolf/dog popun excluding HW/GW from India
   Number of Average substitutions     4.5+2.23 7.4+3.2 7.6+3.4   3.1+1.3
   Miminum-maximum substitutions     0-10 0-15 0-15   0-5
   Median (Mode) substitutions     4 (4) 8 (7) 8 (7)   3 (3)