Figure 5

The Dgt locus is contained within two overlapping BAC clones. The comparative map shows MEST positions on tomato chromosome 1 (LeCHR1) based on microsynteny. Orientation is denoted by arrowheads on each chromosome. Arrows from LeCHR1 to the Arabidopsis MSRs represent BLASTN matches of tomato RFLP markers and BAC insert ends, whereas arrows from MSR1 to LeCHR1 indicate MESTs confirmed by CAPS analysis. See the legends for Table 1 and Figure 2 for MEST abbreviations. The order of MESTs is predicted by the order of Arabidopsis genes in the MSRs, DNA blot analysis, and sequence analysis (see Figure 4). On the basis of this comparative map, the Dgt gene would be located between TC85079 and TC98260 on BAC 93O2 and/or 52M1. Note that the scale bar represents Arabidopsis chromosome physical length.