Figure 3
From: Identification of Schistosoma mansonigender-associated gene transcripts by cDNA microarray profiling
Identification of 28 differentially expressed gender-associated gene products in the adult schistosome by cDNA microarray analysis. Adult male and female cDNA samples were used to probe five schistosome cDNA microarrays. Those 23 arrayed cDNA clones thatpassed the iterative filtering process (described in Materials and methods) were described as being differentially expressed in a gender-dependent manner. Among the 23 differentially expressed cDNAs, 12 novel female associations and 4 new male associations were identified. (a) The 23 cDNAs demonstrating gender-associated gene-expression profiles were plotted away from the line of identity (equal expression between sexes) on a log2-based scale. Clone identifiers (GenBank accession numbers) and common gene names (as annotated by database homologies/identities) define each differentially transcribed cDNA. cDNA clones without common gene names have no known database homologies. Those clones chosen for independent verification by RT-PCR analysis are indicated by gray shading (AI110935, R95590, N21941, N21956, AI111017, AA559678, and R95512). Common gene names indicated with an asterisk (chorion, p48 and mucin-like protein) define the positive control female-associated clones printed on the cDNA microarrays. Mm, Mus musculus; Rn, Rattus norvegicus; Sm, Schistosoma mansoni; Sj, Schistosoma japonicum. These 23 differentially expressed cDNAs hybridized to DNA elements located in every subarray and maintained this characteristic when the fluorochromes were interchanged. (b) Male cDNA labeled with Cy5 compared with Cy3-labeled female cDNA. R95332 (green box) and AI111005 (yellow box) are cDNA clones that were deposited in two different subarrays as an additional quality-control measure. (c)Male cDNA labeled with Cy3 compared with Cy5-labeled female cDNA. cDNA elements indicated by orange circles and arrows are associated with differential expression in the adult male schistosome. cDNA elements indicated by white circles and arrows are associated with differential expression in the adult female.